ibidi Material Specifications
ibidi Surfaces and Coatings
ibidi Polymer Coverslip
 ibiTreat (Tissue Culture-Treated) Surface Excellent adhesion of adherent cells |
 Hydrophobic, Uncoated Surface Weak adhesion of adherent cells, application of specific coatings |
 Bioinert Surface No adhesion of adherent cells |
 µ-Patterned Surface Spatially defined adhesion of adherent cells on spots, different spot geometries available |
 Elastically Supported Surface (ESS) Culture of adherent cells on an elastic surface |
 Coated Surface Culture of adherent cells on a Collagen I, IV or Poly-L-Lysine surface |
Glass Coverslip
 Glass Surface Adhesion of adherent cells (coating might be required), special microscopy applications |
ibidi Bottom Material Specifications
|
|
|
Standard polystyrene plates & dishes |
|||
| Bottom thickness |
180 µm
(+10/–5 µm) |
170 µm
(+/–5 µm) |
170 µm
(+20/–10 µm) |
1000 µm
(+/–50 µm) |
500–1000 µm
|
| Bottom material |
Polymer
|
D 263 M Schott high precision glass
|
D 263 M Schott high precision glass
|
Soda-lime glass
|
Polymer
|
| Surface modifications |
Uncoated glass
|
Uncoated glass
|
Tissue culture-treated,
Untreated |
Compatibility With Microscopy Applications |
|||||
|
|
|
Standard polystyrene plates & dishes |
|||
| Brightfield Microscopy |
++
|
++
|
++
|
++
|
++
|
| Phase Contrast |
++
|
++
|
++
|
++
|
++
|
| Differential Interference Contrast (DIC) |
++
|
++
|
++
|
++
|
-
|
| Widefield Fluorescence |
++
|
++
|
++
|
++
|
-
|
| Confocal Fluorescence |
++
|
++
|
++
|
++
|
-
|
| Two-Photon and Mulitphoton Microscopy |
++
|
++
|
++
|
++
|
-
|
| Fluorescence Recovery After Photobleaching (FRAP) |
++
|
++
|
++
|
++
|
-
|
| Förster Resonance Energy Transfer (FRET) |
++
|
++
|
++
|
++
|
-
|
| Fluorescence Lifetime Imaging Microscopy (FLIM) |
++
|
++
|
++
|
++
|
-
|
| Lightsheet Fluorescence Microscopy (LSFM, SPIM) |
++
|
++
|
++
|
++
|
-
|
| Total Internal Reflection Fluorescence (TIRF) |
+
|
++
|
+
|
+
|
-
|
| Super-Resolution Microscopy |
+
|
++
|
+
|
+
|
-
|
Optical Aspects |
|||||
|
|
|
Standard polystyrene plates & dishes |
|||
| Refractive index (nD 589 nm) |
1.52
|
1.52
|
1.52
|
1.52
|
1.58
|
| Abbe number |
56
|
55
|
55
|
64
|
31
|
| Autofluorescence |
Low
|
Low
|
Low
|
Low
|
Very high
|
| Transmission |
Very high
(even ultraviolet) |
High
(ultraviolet restrictions) |
High
(ultraviolet restrictions) |
High
(ultraviolet restrictions) |
High
(ultraviolet restrictions) |
Further Aspects |
|||||
|
|
|
Standard polystyrene plates & dishes |
|||
| Gas permeability |
Yes
|
No
|
No
|
No
|
Yes
|
| Material flexibility |
High
|
Low
|
Low
|
Very low
|
High
|
| Breakable |
No
|
Yes
|
Yes
|
Yes
|
No
|
| Compatibility with protein coatings |
Yes
|
Yes
|
Yes
|
Yes
|
Yes
|
| Birefringence (DIC) |
Low
(DIC-compatible) |
Low
(DIC-compatible) |
Low
(DIC-compatible) |
Low
(DIC-compatible) |
Very high
|
| Immersion oil compatibility |
Yes
|
Yes
|
Yes
|
Yes
|
No
|
| Correct thickness for high resolution microscopy objective lenses |
Yes
|
Yes
|
Yes
|
No (Yes, if mounted with a standard glass coverslip)
|
No
|
* With the 3 Well | 8 Well | 12 Well Chamber Slide, removable, ibidi provides self-adhesive, removable silicone chambers that are mounted on a standard glass slide. They are suitable for upright and inverted microscopy and enable long-term storage of samples after immunofluorescence staining.