Comparison of Material Specifications

#1.5 ibidi Polymer Coverslip

#1.5H ibidi Glass Coverslip

#1.5 glass
coverslip

Standard
glass slide*

Standard polystyrene plates & dishes

Bottom thickness
180 µm
(+10/–5 µm)
170 µm
(+/–5 µm)
170 µm
(+20/–10 µm)
1000 µm
(+/–50 µm)
500-1000 µm
Bottom material
Polymer
D 263 M Schott high precision glass
D 263 M Schott high precision glass
Soda-lime glass
Polymer
Surface modifications
ibiTreat (tissue culture-treated)
Uncoated (hydrophobic)
Uncoated glass
Uncoated glass
Uncoated glass
tissue culture-treated
Untreated

Applications

#1.5 ibidi Polymer Coverslip

#1.5H ibidi Glass Coverslip

#1.5 glass
coverslip

Standard
glass slide*

Standard polystyrene plates & dishes

Brightfield Microscopy
++
++
++
++
++
Phase Contrast
++
++
++
++
++
Differential Interference Contrast (DIC)
++
++
++
++
-
Widefield Fluorescence
++
++
++
++
-
Confocal Fluorescence
++
++
++
++
-
Two-Photon and Mulitphoton Microscopy
++
++
++
++
-
Fluorescence Recovery After Photobleaching (FRAP)
++
++
++
++
-
Förster Resonance Energy Transfer (FRET)
++
++
++
++
-
Fluorescence Lifetime Imaging Microscopy (FLIM)
++
++
++
++
-
Lightsheet Fluorescence Microscopy (LSFM, SPIM)
++
++
++
++
-
Total Internal Reflection Fluorescence (TIRF)
+
++
+
+
-
Super-Resolution Microscopy
+
++
+
+
-

Optical Aspects

#1.5 ibidi Polymer Coverslip

#1.5H ibidi Glass Coverslip

#1.5 glass
coverslip

Standard
glass slide*

Standard polystyrene plates & dishes

Refractive index (nD 589 nm)
1.52
1.52
1.52
1.52
1.58
Abbe number
56
55
55
64
31
Autofluorescence
Low
Low
Low
Low
Very high
Transmission
Very high
(even ultraviolet)
High
(ultraviolet restrictions)
High
(ultraviolet restrictions)
High
(ultraviolet restrictions)
High
(ultraviolet restrictions)

Further Aspects

#1.5 ibidi Polymer Coverslip

#1.5H ibidi Glass Coverslip

#1.5 glass
coverslip

Standard
glass slide*

Standard polystyrene plates & dishes

Gas permeability
Yes
No
No
No
yes
Material flexibility
High
Low
Low
Very low
High
Breakable
No
Yes
Yes
Yes
No
Compatibility with protein coatings
Yes
Yes
Yes
Yes
Yes
Birefringence (DIC)
Low
(DIC-compatible)
Low
(DIC-compatible)
Low
(DIC-compatible)
Low
(DIC-compatible)
Very high
Immersion Oil compatibility
Yes
Yes
Yes
Yes
No
Correct thickness for high resolution microscopy objective lenses
Yes
Yes
Yes
No (Yes, if mounted with a standard glass coverslip)
No


* With the 3 Well | 8 Well | 12 Well Chamber Slide, removable, ibidi provides self-adhesive, removable silicone chambers that are mounted on a standard glass slide. They are suitable for upright and inverted microscopy and enable long-term storage of samples after immunofluorescence staining.