ibidi Material Specifications
ibidi Surfaces and Coatings
ibidi Polymer Coverslip
 ibiTreat (Tissue Culture-Treated) Surface Excellent adhesion of adherent cells |  Hydrophobic, Uncoated Surface Weak adhesion of adherent cells, application of specific coatings |  Bioinert Surface No adhesion of adherent cells |
 µ-Patterned Surface Spatially defined adhesion of adherent cells on spots, different spot geometries available |  Elastically Supported Surface (ESS) Culture of adherent cells on an elastic surface |  Coated Surface Culture of adherent cells on a Collagen I, IV or Poly-L-Lysine surface |
Glass Coverslip
 Glass Surface Adhesion of adherent cells (coating might be required), special microscopy applications |
ibidi Bottom Material Specifications
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| Standard polystyrene plates & dishes | |||
| Bottom thickness | 180 µm (+10/–5 µm) | 170 µm (+/–5 µm) | 170 µm (+20/–10 µm) | 1000 µm (+/–50 µm) | 500–1000 µm |
| Bottom material | Polymer | D 263 M Schott high precision glass | D 263 M Schott high precision glass | Soda-lime glass | Polymer |
| Surface modifications | Uncoated glass | Uncoated glass | Tissue culture-treated, Untreated |
Compatibility With Microscopy Applications | |||||
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| Standard polystyrene plates & dishes | |||
| Brightfield Microscopy | ++ | ++ | ++ | ++ | ++ |
| Phase Contrast | ++ | ++ | ++ | ++ | ++ |
| Differential Interference Contrast (DIC) | ++ | ++ | ++ | ++ | - |
| Widefield Fluorescence | ++ | ++ | ++ | ++ | - |
| Confocal Fluorescence | ++ | ++ | ++ | ++ | - |
| Two-Photon and Mulitphoton Microscopy | ++ | ++ | ++ | ++ | - |
| Fluorescence Recovery After Photobleaching (FRAP) | ++ | ++ | ++ | ++ | - |
| Förster Resonance Energy Transfer (FRET) | ++ | ++ | ++ | ++ | - |
| Fluorescence Lifetime Imaging Microscopy (FLIM) | ++ | ++ | ++ | ++ | - |
| Lightsheet Fluorescence Microscopy (LSFM, SPIM) | ++ | ++ | ++ | ++ | - |
| Total Internal Reflection Fluorescence (TIRF) | + | ++ | + | + | - |
| Super-Resolution Microscopy | + | ++ | + | + | - |
Optical Aspects | |||||
|
| Standard polystyrene plates & dishes | |||
| Refractive index (nD 589 nm) | 1.52 | 1.52 | 1.52 | 1.52 | 1.58 |
| Abbe number | 56 | 55 | 55 | 64 | 31 |
| Autofluorescence | No | No | No | No | Very high |
| Transmission | Very high (even ultraviolet) | High (ultraviolet restrictions) | High (ultraviolet restrictions) | High (ultraviolet restrictions) | High (ultraviolet restrictions) |
Further Aspects | |||||
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| Standard polystyrene plates & dishes | |||
| Gas permeability | Yes | No | No | No | Yes |
| Material flexibility | High | Low | Low | Very low | High |
| Breakable | No | Yes | Yes | Yes | No |
| Compatibility with protein coatings | Yes | Yes | Yes | Yes | Yes |
| Birefringence (DIC) | Low (DIC-compatible) | Low (DIC-compatible) | Low (DIC-compatible) | Low (DIC-compatible) | Very high |
| Immersion oil compatibility | Yes | Yes | Yes | Yes | No |
| Correct thickness for high resolution microscopy objective lenses | Yes | Yes | Yes | No (Yes, if mounted with a standard glass coverslip) | No |
* With the 3 Well | 8 Well | 12 Well Chamber Slide, removable, ibidi provides self-adhesive, removable silicone chambers that are mounted on a standard glass slide. They are suitable for upright and inverted microscopy and enable long-term storage of samples after immunofluorescence staining.