ibidi Material Specifications
ibidi Surfaces and Coatings

ibidi Polymer Coverslip
![]() ibiTreat (Tissue Culture-Treated) Surface Excellent adhesion of adherent cells |
![]() Hydrophobic, Uncoated Surface Weak adhesion of adherent cells, application of specific coatings |
![]() Bioinert Surface No adhesion of adherent cells |
![]() µ-Patterned Surface Spatially defined adhesion of adherent cells on spots, different spot geometries available |
![]() Elastically Supported Surface (ESS) Culture of adherent cells on an elastic surface |
![]() Coated Surface Culture of adherent cells on a Collagen I, IV or Poly-L-Lysine surface |

Glass Coverslip
![]() Glass Surface Adhesion of adherent cells (coating might be required), special microscopy applications |
ibidi Bottom Material Specifications
|
|
Standard polystyrene plates & dishes |
|||
Bottom thickness |
180 µm
(+10/–5 µm) |
170 µm
(+/–5 µm) |
170 µm
(+20/–10 µm) |
1000 µm
(+/–50 µm) |
500–1000 µm
|
Bottom material |
Polymer
|
D 263 M Schott high precision glass
|
D 263 M Schott high precision glass
|
Soda-lime glass
|
Polymer
|
Surface modifications |
Uncoated glass
|
Uncoated glass
|
Tissue culture-treated,
Untreated |
Compatibility With Microscopy Applications |
|||||
|
|
Standard polystyrene plates & dishes |
|||
Brightfield Microscopy |
++
|
++
|
++
|
++
|
++
|
Phase Contrast |
++
|
++
|
++
|
++
|
++
|
Differential Interference Contrast (DIC) |
++
|
++
|
++
|
++
|
-
|
Widefield Fluorescence |
++
|
++
|
++
|
++
|
-
|
Confocal Fluorescence |
++
|
++
|
++
|
++
|
-
|
Two-Photon and Mulitphoton Microscopy |
++
|
++
|
++
|
++
|
-
|
Fluorescence Recovery After Photobleaching (FRAP) |
++
|
++
|
++
|
++
|
-
|
Förster Resonance Energy Transfer (FRET) |
++
|
++
|
++
|
++
|
-
|
Fluorescence Lifetime Imaging Microscopy (FLIM) |
++
|
++
|
++
|
++
|
-
|
Lightsheet Fluorescence Microscopy (LSFM, SPIM) |
++
|
++
|
++
|
++
|
-
|
Total Internal Reflection Fluorescence (TIRF) |
+
|
++
|
+
|
+
|
-
|
Super-Resolution Microscopy |
+
|
++
|
+
|
+
|
-
|
Optical Aspects |
|||||
|
|
Standard polystyrene plates & dishes |
|||
Refractive index (nD 589 nm) |
1.52
|
1.52
|
1.52
|
1.52
|
1.58
|
Abbe number |
56
|
55
|
55
|
64
|
31
|
Autofluorescence |
Low
|
Low
|
Low
|
Low
|
Very high
|
Transmission |
Very high
(even ultraviolet) |
High
(ultraviolet restrictions) |
High
(ultraviolet restrictions) |
High
(ultraviolet restrictions) |
High
(ultraviolet restrictions) |
Further Aspects |
|||||
|
|
Standard polystyrene plates & dishes |
|||
Gas permeability |
Yes
|
No
|
No
|
No
|
Yes
|
Material flexibility |
High
|
Low
|
Low
|
Very low
|
High
|
Breakable |
No
|
Yes
|
Yes
|
Yes
|
No
|
Compatibility with protein coatings |
Yes
|
Yes
|
Yes
|
Yes
|
Yes
|
Birefringence (DIC) |
Low
(DIC-compatible) |
Low
(DIC-compatible) |
Low
(DIC-compatible) |
Low
(DIC-compatible) |
Very high
|
Immersion oil compatibility |
Yes
|
Yes
|
Yes
|
Yes
|
No
|
Correct thickness for high resolution microscopy objective lenses |
Yes
|
Yes
|
Yes
|
No (Yes, if mounted with a standard glass coverslip)
|
No
|
* With the 3 Well | 8 Well | 12 Well Chamber Slide, removable, ibidi provides self-adhesive, removable silicone chambers that are mounted on a standard glass slide. They are suitable for upright and inverted microscopy and enable long-term storage of samples after immunofluorescence staining.