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µ-Slide 8 Well
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8 Well Chamber, removable
Chamber slide with a removable 8 well silicone chamber for cell culture and immunofluorescence staining
- The ideal chambered slide for cost-effective immunofluorescence assays and long-term sample storage
- Suitable for use with upright and inverted microscopes
- Large growth area combined with a small staining volume (especially 3 Well Chamber, removable)
Product Details
Applications:
- Immunofluorescence assays using standard coverslip techniques
- Long-term sample storage
- 8 fold sample preparation of adherent cells and tissue samples
Specifications:
| Number of wells | 8 |
| Dimensions of wells (w x l x h) in mm | 12 x 7.75 x 8.0 |
| Volume per well | 400 µl |
| Growth area per well | 0.93 cm² |
| Coating area per well | 2.63 cm² |
| Material | Biocompatible silicone |
| Suitable coverslip size | 24 mm x 60 mm |
| Bottom | Glass slide (26 x 76 x 1 mm) |
Technical Features:
- Self-adhesive silicone gasket mounted on a standard glass slide
- Biocompatible silicone material
- Non-fluorescent glass slide with frosted ends for easy handling and labeling
- Suitable for use with upright and inverted microscopes, after staining and mounting with a coverslip
- Compatible with all common mounting media
- May require coating to promote cell attachment
- Optional: The gasket may be transferred to any flat clean surface for cell cultivation
Standard Procedure 3 Well | 8 Well | 12 Well Chamber, removable:
Staining Protocol:
Supporting Material
Instructions
Product Flyer
FAQ
- Which mounting medium is recommended for mounting glass slides and coverslips in the Immunofluorescence Chambers 3 Well | 8 Well | 12 Well Chamber, removable?
- Can the ibidi Mounting Medium be used with the Immunofluorescence Chambers 3 Well | 8 Well | 12 Well Chamber, removable?
- Is the silicon gasket of the Immunofluorescence Chambers 3 Well | 8 Well | 12 Well Chamber, removable reusable?
- µ-Slides and µ-Dishes
- Which solvents and chemicals are compatible with the ibidi labware?
User Comments
Milena Leseva, The Stephan Angeloff Institute of Microbiology, Sofia, Bulgaria
"The 12 Well and 8 Well Chambers, removable worked very well. We used them for fluorescence staining for H4K16ac in non-adherent, neutrophil cells. We treated the cells with different compounds that are expected to change this modification.
Overall, we are extremely happy with how easy it is to use the cell culture chambers, especially when you have cells with different treatments. We liked the fact that the chamber format minimizes the volume of antibody that we required in order to get a good signal."
Milena Leseva
The Stephan Angeloff Institute of Microbiology
Sofia
Bulgaria
http://microbio.bas.bg/wordpress/index.php/en/
Petra Weber, Aalen University, Optical Engineering, Aalen, Germany
"We have tested the Removable Chambers (3 Well and 8 Well) and were very happy. Due to our very sensitive TIRF microscopy with an upright microscope we need appropriate glass slides with removable chambers. We tested adherent HeLa cells to detect Förster Resonance Energy Transfer between EGFR-CFP and Grb2-YFP with Fluorescence Lifetime Imaging. In particular for the activation or inhibition of intracellular signaling pathways, we need the subdivisions on the object slide. It was very easy to remove the chambers. Thank you very much for the possibility to test the chambers for free. I am sure I will use them again."
Petra Weber
Aalen University, Optical Engineering
Aalen, Germany
https://www.hs-aalen.de/de
Daniel Kummer, ZMBE, Institute for Medical Biochemistry, Münster, Germany
"I tested the 8 Well Chamber, removable, and was very happy with the results. The system worked flawlessly. The cells grew very well and the immunofluorescence stainings were of good quality. In general, our institute has always had good experiences with ibidi products. It was the same with the 8 Well Chamber, removable. I have already recommended it to my colleagues."
Daniel Kummer
ZMBE, Institute for Medical Biochemistry
Münster, Germany
