Cross Section of the Channel: Same Slide – Different Channel Height and Volume
* not recommended for static culture over more than 6 hours
Choosing the Right Channel for Flow Applications
For flow assays with small amounts of medium and high values of shear stress
0.2 mm channel
For a wide range of shear stress
0.4 mm channel
For controlling low values of shear stress (<2 dyne /cm²)
0.6 and 0.8 mm channels
Static Cultures vs. Flow Applications
The general rule is: Low channels are more suitable for flow applications. High channels are more suitable for static cell culture.
Select the ideal Perfusion Set for your flow application here.
Compatible with Solvents for Staining and Fixation
HUVEC, cultivated over 7 days at 10 dyn / cm². VE-cadherins are stained in green, cell nuclei are stained in blue.
Influence of Shear Stress on Cultured Cells
Human umbilical vein endothelial cells (HUVEC) cultured under flow conditions (20 dyn / cm²) in a µ-Slide I 0.4 Luer over 9 days. The primary cells were transduced with the adenoviral vector rAV CMV-LifeAct-TagRFP 24 hours prior to the experiment.
"Thank you for the great products that you have developed over the years. The ibidi Pump System has immeasurably helped our research. We have recently published a paper in “Stem Cells” that uses your flow system, the µ-Slide I Luer, and the µ-Plate 96 Well very effectively. It seems to me that ibidi are constantly coming up with clever and useful products, and I like to keep an eye on what interesting things are coming out next from them."
Shane R. McSweeney, Cardiovascular Division, BHF Centre of Research Excellence, School of Medicine, King’s College London, United Kingdom
"The µ-Slide I Luer facilitates the culture of endothelial cells under defined complex flow patterns. We are particularly interested in the effects of oscillatory shear stress on redox signaling pathways, and the ibidi Air Pump and Fluidic Units are ideal systems for this purpose. The µ-Slides are convenient for both live and fixed cell imaging as well as the collection of protein and RNA samples and other cell analyses. The system has allowed us to easily investigate physiological shear patterns in vitro without a background in engineering.”
Shane R. McSweeney Cardiovascular Division BHF Centre of Research Excellence School of Medicine King’s College London United Kingdom
Dr. Thomas A.J. McKinnon, Imperial College London, UK
„I work with the ibidi slides because they are superior to any other product of this kind on the market. They are easy to use, give consistent results, economical and are suitable for a wide range of applications. The flow slides have without a doubt transformed my labs research and made many new experiments possible. Well done ibidi! I am indeed extremely happy with the ibidi products.”
Dr. Thomas A.J. McKinnon BSc, PhD Post Doctoral Research Associate Department of Hematology Imperial College London UK