Application Notes | General Cell Culture
AN 03: Cell Culture in ibidi Channel Slides: Using the µ-Slide VI 0.4 as an Example (PDF)
Benefits and handling of the µ-Slide VI 0.4, and a comparison between channel slides and slides with open wells
AN 06: Cultivation and Detachment of Adherent Cells in the µ-Slide VI 0.4 (PDF)
Removing adherently grown cells from a µ-Channel after cultivation
AN 07: Transfection (PDF)
Example showing how protocols for transfection can easily be adapted to the work in cell culture channels
AN 08: Coating Protocols for ibidi Labware (PDF)
A detailed protocol on how to coat the ibidi labware (e.g., with Collagen, Fibronectin, or Poly-L-Lysine) including required volumes and protein concentrations
AN 10: Co-Cultivation (PDF)
Application note for co-cultivation of two different cell types in µ-Slide 2 Well Co-Culture
AN 12: Avoiding Evaporation: Humidity Control in Cell Culture (PDF)
An overview of different methods to avoid evaporation from cell culture vessels by controlling the humidity
AN 20: Cultivation of Macrophages (PDF)
Cultivation protocol for a murine macrophage cell line in µ-Slide VI 0.4 and µ-Slide 8 Well
AN 65: Cell Adhesion on ibidi μ-Patterns: Parameters and Optimization (PDF)
A detailed protocol on how to test and optimize cell adhesion on the ibidi µ-Patterns
AN 03: Cell Culture in ibidi Channel Slides: Using the µ-Slide VI 0.4 as an Example (PDF)
Benefits and handling of the µ-Slide VI 0.4, and a comparison between channel slides and slides with open wells
AN 06: Cultivation and Detachment of Adherent Cells in the µ-Slide VI 0.4 (PDF)
Removing adherently grown cells from a µ-Channel after cultivation
AN 07: Transfection (PDF)
Example showing how protocols for transfection can easily be adapted to the work in cell culture channels
AN 09: Fluorescence Staining Using a µ-Slide VI (PDF)
Examples describing how to do immunofluorescence stainings using µ-Slides
AN 15: Fluorescence Staining Using the µ-Slide y-shaped (PDF)
Examples describing how to do immunofluorescence stainings using µ-Slides
AN 25: Serial Connection of Luer-Slides for Flow Experiments (PDF)
Step-by-step protocol for how to create a serial connection between two µ-Slide I 0.6 Luer channels
AN 69: Serial Connection of μ-Slide Spheroid Perfusion Channels for Long-Term Cultivation of 3D Spheroids Under Flow (PDF)
A detailed protocol to connect multiple µ-Channel Slides in series using one Pump System