Image of the Month
2023 ibidi Calendar, December
Ana Kriselj
Jozef Stefan Institute, Ljubljana, Slovenia
Human microglial cells (HMC3) were stained with Hoechst (nuclei, green) and ATTO 647N DPPE (red), a lipophilic fluorescent probe. After incorporation of the phospholipid into the plasma membrane, the fluorophore is located at the water/lipid interface of the membrane. Over time, it is incorporated into the cell, labeling further structures with lipid membranes. The image was acquired in a μ-Slide 8 Well high Glass Bottom using super-resolution (STED) mounted on an Olympus IX83 microscope with a 60x objective lens.
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