Designed for specific uses (e.g., gradients or gel matrices)
Your cell-based assays are as versatile as our chambers: We provide imaging chambers with various geometries that are specifically tailored to your assay. In order to guarantee the highest imaging quality at optimized experimental conditions, we have developed our imaging chambers using a combination of geometrical patterns and physical features. The all-in-one chambers reduce the time and number of experimental steps needed in cell-based assays. In addition, ibidi provides labware with specialized geometrical designs for functional cell-based assays, such as angiogenesis, chemotaxis, wound healing, or cell migration assays.
All-In-One Chambers: All Steps in One Single Slide
All-in-one chambers have been developed to optimize the daily lab routine by providing an all-in-one solution for cell cultivation, treatment, and live cell imaging. The design of the ibidi μ-Slides, μ-Dishes, and μ-Plates allows for all of the experimental steps—from cell cultivation to microscopic imaging—to be conducted in one single chamber.
Channel formats like the μ-Slide VI 0.4 are ideal for an exact exchange of small amounts of reagents. These μ-Slides reduce the number of experimental steps needed for immunofluorescence assays. Due to the low volume, the channel slides help saving a lot of money for antibodies.
The ibidi all-in-one chambers reduce the number of experimental steps needed in immunofluorescence assays.
Order a free sample to test the ibidi µ-Slides, µ-Dishes, and µ-Plates with your experiments.
Excurse: Chamber Slides, Removable
With the 3 Well | 8 Well | 12 Well Chamber Slides, removable, ibidi provides self-adhesive, removable silicone chambers that are mounted on a standard glass slide (1 mm thickness). They are suitable for upright and inverted microscopy and enable long-term storage of samples after immunofluorescence staining.
All steps in the immunofluorescence assay can be done in one single slide, which simplifies the overall procedure and requires only a small number of cells and a low amount of antibody and medium.
Please note: In principle, glass bottoms are suitable for direct cell culture. However, to promote cell attachment, a surface coating might be required prior to cell seeding.
Immunofluorescence using the 12 Well Chamber, removable
The ibidi Chamber Slides, removable, allow for cell cultivation (top) and upright microscopy of cells (bottom) after immunofluorescence staining.
Triple immunofluorescence of Madin-Darby canine kidney (MDCK) cells. Red: mitochondria, stained with MitoTracker™ Red CMXRos; Green: F-actin, stained with Alexa Fluor™ 488 Phalloidin; Blue: nuclei, stained with DAPI.
Channel Slides: Homogenous Cell Growth and Optimized Phase Contrast Microscopy
Homogeneous Cell Distribution
In addition to being optimized for cell culture under flow, the ibidi channel slides format provides the ideal geometry for homogeneous cell distribution.
Cell densities in open wells are very dependent on handling during cell seeding. Due to the closed channel geometry, cell densities in channels do not vary depending on their positions inside the slide, or with their handling and treatment during and after cell seeding.
To demonstrate the influence of slide geometry on cell distribution, cultivated cells were visualized macroscopically and microscopically in the open µ-Slide 8 Well and the channel μ-Slide VI 0.4, respectively. Macroscopically, the cells cultured in the µ-Slide 8 Well formed characteristic patterns. In general, the cell density in the middle of the well reached its maximum, while fewer cells attached to the outer well regions. Some cells congregated to the edges of the well. In contrast, the cell distribution in the μ-Slide VI 0.4 stayed homogeneous. This was confirmed by phase contrast and fluorescence microscopy.
µ-Slide 8 Well
µ-Slide VI 0.4
Geometric scheme and cell distribution in the µ-Slide 8 Well (left) and the µ-Slide VI 0.4 (right). Cells were visualized macroscopically using crystal violet staining (top), and microscopically using phase contrast (middle) and fluorescence microscopy (bottom).
No Lid—No Condensation
Condensation, which occurs on the lid of the culture vessel after being removed from the incubator, is another effect that disrupts phase contrast microscopy. When using the ibidi channel slides, condensation inside the optical pathway is intrinsically impossible.
Optimized Phase Contrast Microscopy
The meniscus formation in open well chambers at the air-water-interphase is a natural and unavoidable effect that can disturb phase contrast microscopy. In channel slides (e.g., the ibidi µ-Slide VI 0.4), meniscus formation is geometrically impossible. Therefore, phase contrast in channel slides is superior to small open wells.
Find more detailed information about the principle of phase contrast microscopy and ibidi labware solutions here.
96 well plate or small open well: Strong influence of the meniscus, low contrast near the edges.
Channel or parallel plates: No meniscus, good phase contrast over the entire area.
The µ-Slides and µ-Dishes with Ph+ feature (Phase Contrast +) are designed for excellent phase contrast microscopy without meniscus, which naturally disturbs the phase contrast effect in standard open wells. The Ph+ version provides a special intermediate plate in the center of the well. This plate flattens the meniscus and creates a parallel beam throughout the plate, thereby increasing the area of well-contrasted cells. Openings near the corners provide access to the wells for the easy filling and aspiration of liquids. This innovative technique supports meniscus-free phase contrast microscopy in a very convenient manner.
Depending on your application, you can choose between the following labware formats:
Illustration of the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.