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µ-Slide VI 0.4

µ-Slide VI 0.4 View larger

A 6 channel μ-Slide suitable for flow experiments and for immunofluorescence assays

  • All-in-one chamber that simplifies immunofluorescence protocols
  • Homogeneous cell distribution over the channel surface, regardless of handling practices
  • Cost-effective experiments with small numbers of cells and low volumes of reagents

More details

Product Details

Applications

Specifications

Outer dimensions (w x l) 75.5 x 25.5 mm
Adapters Female Luer
Number of channels 6
Channel volume 30 µl
Channel height 0.4 mm
Channel length 17 mm
Channel width 3.8 mm
Volume per reservoir 60 µl
Growth area 0.6 cm² per channel
Coating area using 30 µl 1.2 cm² per channel
Bottom: ibidi Polymer Coverslip

Define and print your
experimental setup

Technical Features

  • 30 µl channel volume, which saves on reagent consumption
  • Easy connection to existing tubes and pumps via female Luer adapter
  • Fully compatible with high resolution fluorescence microscopy
  • Defined shear stress and shear rate level

All-in-One-Chamber for Fast Immunofluorescence Protocols

Optimal Cell Growth in the µ-Slide VI 0.4

The entire observation field is in excellent phase contrast.

Experimental Examples

Super-Resolution Microscopy (STED) of the Actin Cytoskeleton

The µ-Slide VI 0.4 is compatible with super-resolution microscopy methods, such as simulated emission depletion (STED) microscopy. Using LifeAct-TagGFP2 Protein, the actin cytoskeleton can be visualized in detail. In this experiment, fixed Rat1 fibroblasts were incubated with LifeAct-TagGFP2 protein in a µ-Slide VI 0.4, ibiTreat. STED microscopy was performed to create a super-resolution image.


Super-resolution microscopy of the actin cytoskeleton in Rat1 fibroblasts using LifeAct-TagGFP2 Protein. Microscopy was performed on the STEDYCON super-resolution STED nanoscopy system (Abberior Instruments GmbH, Göttingen, Germany) with a Plan-Neofluar 100x/1.4 objective lens.

Fluorescence Microscopy for Mitochondria Visualization

The µ-Slide VI 0.4 is compatible with a variety of fluorescence microscopy methods. In this experiment, Madin-Darby Canine Kidney (MDCK) cells were cultured in a µ-Slide VI 0.4 and their mitochondria were visualized using MitoTracker.


Fluorescence microscopy of MDCK cells cultured in a µ-Slide VI 0.4. Mitochondria (MitoTracker, red), Actin cytoskeleton (Phalloidin, green), nuclei (DAPI, blue).

Supporting Material

Movies

User Comments

Dr. Thomas A.J. McKinnon, Imperial College London, UK

„I work with the ibidi slides because they are superior to any other product of this kind on the market. They are easy to use, give consistent results, economical and are suitable for a wide range of applications. The flow slides have without a doubt transformed my labs research and made many new experiments possible. Well done ibidi! I am indeed extremely happy with the ibidi products.”

Dr. Thomas A.J. McKinnon BSc, PhD
Post Doctoral Research Associate
Department of Hematology
Imperial College London
UK

http://www.imperial.nhs.uk/hammersmith/

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