µ-Slide VI 0.4

Applications

• Immunofluorescence assays and live cell imaging
• Real-time imaging under either static or flow conditions
• Parallel screenings using multichannel pipettes

Specifications

Define and print your experimental setup

Technical Features

• 30 µl channel volume, which saves on reagent consumption
• Easy connection to existing tubes and pumps via female Luer adapter
• Fully compatible with high resolution fluorescence microscopy
• Defined shear stress and shear rate level

All-in-One-Chamber for Fast Immunofluorescence Protocols

Optimal Cell Growth in the µ-Slide VI ^0.4

The entire observation field is in excellent phase contrast.

Experimental Examples

Super-Resolution Microscopy (STED) of the Actin Cytoskeleton

The µ-Slide VI ^0.4 is compatible with super-resolution microscopy methods, such as simulated emission depletion (STED) microscopy. Using LifeAct-TagGFP2 Protein, the actin cytoskeleton can be visualized in detail. In this experiment, fixed Rat1 fibroblasts were incubated with LifeAct-TagGFP2 protein in a µ-Slide VI ^0.4, ibiTreat. STED microscopy was performed to create a super-resolution image.

Super-resolution microscopy of the actin cytoskeleton in Rat1 fibroblasts using LifeAct-TagGFP2 Protein. Microscopy was performed on the STEDYCON super-resolution STED nanoscopy system (Abberior Instruments GmbH, Göttingen, Germany) with a Plan-Neofluar 100x/1.4 objective lens.

Fluorescence Microscopy for Mitochondria Visualization

The µ-Slide VI ^0.4 is compatible with a variety of fluorescence microscopy methods. In this experiment, Madin-Darby Canine Kidney (MDCK) cells were cultured in a µ-Slide VI ^0.4 and their mitochondria were visualized using MitoTracker.

Fluorescence microscopy of MDCK cells cultured in a µ-Slide VI ^0.4. Mitochondria (MitoTracker, red), Actin cytoskeleton (Phalloidin, green), nuclei (DAPI, blue).