Explore the wide range of advanced microscopy techniques used in cell biology and biomedical research, from phase contrast and widefield fluorescence to cutting-edge methods like confocal, multiphoton, and super-resolution microscopy. This ibidi Application Chapter highlights the principles and applications of each technique—including DIC, TIRF, FRAP, FLIM, LSFM, and FRET—helping you choose the best imaging method for your scientific needs.