Use the ibidi flow calculation tools to precisely determine the wall shear stress and the shear rate in your microfluidic or perfusion-based experiments. With key parameters—such as flow rate, fluid viscosity, and channel geometry—you can define and control the mechanical environment experienced by an adherent cell monolayer on the inner wall of your flow channel.

Shear Stress Calculator

Wall shear stress is the force generated by flowing medium and acting on the surface of a cell culture channel. Use this calculator to define shear stress in your experiment when flow conditioning of an adherent cell layer on the channel wall is needed.

Shear Rate Calculator

Wall shear rate describes the flow profile at the channel wall. It influences all interactions between the channel wall and cells or particles moving with the fluid flow.

Define the shear rate if you are performing adhesion assays with cells or particles in the medium.

These tools are particularly useful for:

• Simulating physiological shear conditions
• Designing dynamic and automated perfusion-based cell culture assays
• Standardizing experimental conditions for vascular biology, immune cell adhesion, or tumor cell migration under wall shear stress
• Ensure reproducibility and accuracy in your flow experiments with this calculator

While the calculators works with any flow system, they are ideally suited for use with the ibidi Perfusion System and the ibidi Channel Slides to ensure reproducible, controlled, and physiologically relevant shear forces in your experiments.