Cell Culture Under Flow

Experimental Workflow

ibidi Products for this Application

µ-Slide III^3D Perfusion

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Application Guide

Download the whole "Cell Culture Under Flow" Application Guide as a PDF here.

Scientific Posters

Changes in cell-cell contacts of endothelial cells (HUVEC) under long term shear stress conditions (PDF)

Presented at the North American Vascular Biology Organization (NAVBO) Conference 2014, Monterey, California, USA.

Defining the Critical Shear Stress Range in Long Term HUVEC Cell Culture (PDF)

Presented at the 17^th Barrier and Transporter Meeting 2015, Bad Herrenalb, Germany.

Selected Publications

D.C. 't Hart, J. van der Vlag, T. Nijenhuis. Laminar flow substantially affects the morphology and functional phenotype of glomerular endothelial cells. PLoS One, 2021, 10.1371/journal.pone.0251129
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I. Xanthis, C. Souilhol, J. Serbanovic-Canic, et al. β1 integrin is a sensor of blood flow direction. J Cell Sci, 2019, 10.1242/jcs.229542
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J. Xu, J. Mathur, E. Vessières, et al. GPR68 Senses Flow and Is Essential for Vascular Physiology. Cell, 2018, 10.1016/j.cell.2018.03.076, 0092-8674
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T. Keeley, R. Siow, R. Jacob and G. Mann. A PP2A-mediated feedback mechanism controls Ca²⁺-dependent NO synthesis under physiological oxygen. The FASEB Journal, 2017, 10.1096/fj.201700211R
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A. Sabine, E. Bovay, C.S. Demir, et al. FOXC2 and fluid shear stress stabilize postnatal lymphatic vasculature. The Journal of Clinical Investigation, 2015, 10.1172/JCI80454
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