Screening and High Throughput

High Throughput Screening (HTS) With ibidi µ-Plates

Multiwell plates are the gold standard for high throughput screening (HTS) and high content screening (HCS) in cell culture. The ibidi µ-Plates provide essential features that make them ideal for HTS/HCS when using high-resolution microscopy and fluorescence-based imaging:

• Compatible with robotics and plate readers due to an ANSI/SLAS (SBS) standard format (85.5 x 127.5 mm)
• Available with a #1.5 ibidi Polymer Coverslip bottom with extremely low autofluorescence for undisturbed fluorescence microscopy and ibiTreat (tissue culture-treated) surface for optimal cell attachment
• Available with a #1.5H Glass Coverslip bottom with highest optical quality for special microscopic applications (e.g., TIRF, super-resolution microscopy)
• Compatible with solvents for staining and fixation, as well as immersion oil
• Excellent inner well and whole plate flatness

HTS Application Examples for ibidi µ-Plates

• Compound toxicology screenings and drug screenings
• Large-scale transfection experiments
• Large-scale fluorescence microscopy

ibidi Solutions The µ-Plate 24 Well Black ID 14 mm is a microtiter plate with black walls and 24 round wells that have a flat, clear bottom. It is suitable for versatile cell-based assays, such as wound healing experiments.
We offer microtiter plates with black quadratic wells and a flat, clear bottom. They are available with a #1.5 ibidi Polymer Coverslip (µ-Plate 96 Well Black) or a #1.5H Glass Coverslip bottom (µ-Plate 96 Well Black Glass Bottom and µ-Plate 384 Well Black Glass Bottom). The black walls guarantee a low well-to-well crosstalk, which makes the µ-Plate ideal for fluorescence microscopy.

Large-Scale Live Cell Imaging Solutions

Live cell imaging is the time-lapse microscopy of dynamic processes in living cells. Several imaging techniques can be applied, such as phase contrast microscopy or confocal microscopy. During the whole live cell imaging experiment, the cells need to be kept alive and healthy. Therefore, physiological conditions have to be established and maintained on the microscope.

ibidi offers Stage Top Incubation Systems for high throughput live cell imaging using microplates that have an ANSI/SLAS (SBS) standard format.

Stable and consistent temperature distribution in every well of multiwell plates in the ibidi Stage Top Incubation System. Images were acquired with a FLIR thermal camera.

Find Out More Please find more detailed information about the planning, conduction, and data analysis of live cell imaging assays here. Download the whole "Live Cell Imaging" Application Guide as a PDF here.

ibidi Solutions The ibidi Stage Top Incubation Systems allow for easy live cell imaging on every inverted microscope, providing precise control of temperature, humidity, and CO2 (optionally O2). They are compatible with all inverted microscopes that have a K-Frame stage (160 mm x 110 mm) or a Nikon Ti-S-E and Ti-S-ER Motorized Stage.

High Throughput Wound Healing Assays

Wound healing and migration assays are widely used approaches for the analysis of cell migration under different conditions (e.g., when performing drug screenings).
With the Culture-Inserts and the Wound Healing ACAS Image Analysis software, ibidi offers a complete solution for reproducible large-scale wound healing and migration experiments, requiring only a few steps from sample preparation to image analysis:

• Cells are seeded in the single wells, where they attach
• Removal of the silicone insert results in two precisely defined cell patches, which are separated by a 500 µm gap
• Wound closure can now be monitored by using live cell imaging or by taking photos at different time points

Find Out More Please find more detailed information about the planning, conduction, and data analysis of wound healing and migration assays here. Download the whole "Wound Healing and Migration Assays" Application Guide as a PDF here.

ibidi Solutions The ready-to-use Culture-Insert 2 Well 24 is ideal for reproducible high throughput wound healing and migration assays. It consists of silicone Culture-Inserts with a defined cell-free gap that are already inserted into the µ-Plate 24 Well Black. Find a PDF of the detailed protocol for performing a wound healing assay using the Culture-Insert 2 Well 24 in the ibidi Application Note "Wound Healing Assay with the Culture-Insert 2 Well 24" (AN 36).

With the Wound Healing ACAS Image Analysis software, ibidi provides a time-saving, fully automated solution for the quantification of wound healing and migration assays. It includes analyses of the cell-free area, the cell-covered area, and the speed of wound closure.

High Throughput Tube Formation Assays

Tube formation assays are a widely used in vitro tool for accessing angiogenesis in an easy, cost-effective, and reproducible manner. A tube formation assay is performed by first seeding single cells, and then observing and imaging the tube formation over time. From this, several readouts can be analyzed over time, such as tube length and the number of loops.

With the µ-Plate Angiogenesis 96 Well and the Tube Formation ACAS Image Analysis software, ibidi offers a complete solution for tube formation experiments with only a few steps from sample preparation to image analysis. Due to the “well-in-a-well” technology, the amount of gel needed is reduced to only 10 μl per well. In addition, no meniscus is formed, ensuring the formation of a uniformly thick gel matrix.

For scientists who need only a small number of samples, ibidi also offers the µ-Slide Angiogenesis with 15 wells.

Find Out More Please find more detailed information about the planning, conduction, and data analysis of angiogenesis assays here. Download the whole "Angiogenesis" Application Guide as a PDF here.

ibidi Solutions The µ-Plate Angiogenesis 96 Well is a multiwell plate with full ANSI/SLAS (SBS) and robotics compatibility. Due to the well-in-a-well principle, it is ideal for cost-effective tube formation experiments, requiring only 10 µl of gel per well. In addition, it allows for brilliant cell visualization without meniscus formation. Find practical advice on how to run tube formation assays using the µ-Plate Angiogenesis 96 Well in the ibidi Application Note “Tube Formation in µ-Plate Angiogenesis 96 Well” (AN 05) as a PDF.

With the Tube Formation ACAS Image Analysis software, ibidi provides a time-saving, fully-automated solution for the quantification of tube formation assays. It includes analyses of the loop count, average loop area, average tube length (per loop), total tube length, and branch count.

Impedance-Based Wound Healing Assays Using ECIS

With ECIS impedance measurements, functional wound healing and migration assays can be performed with unrivaled convenience. After wounding cells on an electrode with an electric pulse, the ECIS label-free impedance measurement technology allows the user to measure the wound healing process in real time. ECIS impedance assays are label free and are performed inside the cell culture incubator.