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International Cell Culture Under Flow Meeting 2024

July 24–25, 2024 | Northwestern University, Chicago, IL, USA

This meeting took place in the past, the registration is closed.

Chicago

Date:July 24–25, 2024

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Location:logo_northwestern.png
Northwestern University, Chicago, IL, USA
Organizers:Luisa Arispe, Northwestern University, Chicago, IL, USA
Roman Zantl, ibidi, Munich, Germany
Contact:E-mail: flowmeeting2024@ibidi.com
Phone USA: (844) 276-6363
Phone International: +49 89 520 46 17-0

Focus of the Meeting

The second international “Cell Culture Under Flow” meeting brought together scientists from a variety of fields (e.g., vascular research, immunology, barrier function), who cultivate cells under shear stress.

Participants had the chance to present their most recent work. In addition, both young and experienced scientists had the opportunity to network and discuss future objectives of this field of research. Abstracts were selected for oral presentation and posters.

Speakers

  • Hanjoong Jo (Georgia Tech and Emory University, USA)
  • Jan Kitajewski (University of Illinois, USA)
  • Julia Mack (University of California Los Angeles, USA)
  • Matthew Kutys (University of California San Francisco, USA)
  • Paul Evans (Queen Mary University of London, United Kingdom)
  • Simone Bergmann (Technical University Braunschweig, Germany)
  • Tatiana Petrova (University of Lausanne, Switzerland)
  • Yun Fang (University of Chicago, USA)

Impressions of the Meeting

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Program

July 24

Registration and Reception

8:30 am Registration


Welcome and Keynote Lecture

9:30–10:15 am

Chair: Roman Zantl (ibidi GmbH, GER)

9:30 am10 minWelcome:
Luisa Iruela-Arispe (Northwestern University, USA) / Roman Zantl (ibidi GmbH, GER)
9:40 am30 + 5 minKeynote: Flow-Induced Endothelial Polarity
Luisa Iruela-Arispe (Northwestern University, USA)


Session 1:  Microvascular Morphogenesis

10:15–11:05 am

Chair: Roman Zantl (ibidi GmbH, GER)

10:15 am30 + 5 minKeynote: Coordination of Vascular Morphogenesis at Endothelial Cell-Cell InterfacesMatthew Kutys (University of California San Francisco, USA)
10:50 am10 + 5 minOscillatory Shear Stress Modulates Lymphatic Progenitor Cells Maturation Into Lymphatic Vessels With Anti-Cancer PhenotypesNancy Lightsey (University of Notre Dame, USA)
11:05 am35 minCoffee break


Session 2:  Mechanotransduction

11:40 am–1:00 pm

Chair: Paul Evans (Queen Mary University of London, UK)

11:40 am30 + 5 minKeynote: Flow-Mediated Mechanisms of Endothelial Cell ResilienceJulia Mack (University of California Los Angeles, USA)
12:15 pm10 + 5 minLow Shear in Short-Term Impacts Endothelial Cell Traction and Alignment in Long-Term Under High ShearSangyoon Han (Michigan Technological University, USA)
12:30 pm10 + 5 minMechanotransduction via Endothelial Adhesion Molecule CD31 Initiates Transmigration and Reveals a Role for VEGFR2 in DiapedesisBill Muller (Northwestern University, USA)
12:45 pm10 + 5 minCD36-Mediated Lipid Uptake Mediates Endothelial Stiffening Under Disturbed Flow and in Aortic ArchVictor Aguilar (University of Illinois, USA)
1:00 pm60 minLunch break


Session 3:  Molecular Mechanisms of Shear Stress Response 

2:00–3:20 pm

Chair: Tatiana Petrova (University of Lausanne, CH)

2:00 pm30 + 5 minKeynote: Understanding Endothelial Cell Responses to Flow Through Study of Notch Target GenesJan Kitajewski (University of Illinois, USA)
2:35 pm10 + 5 minNotch Signaling Regulates Unc5B to Suppress Endothelial Proliferation and Branching and to Support Junction Formation and Polarization in Response to FlowL.A. Naiche (University of Illinois, USA)
2:50 pm10 + 5 minFlow-sensitive Endothelial K+ Channels are Master Regulators of Endothelial Mechanotransduction and Gene ExpressionSang Joon Ahn (University of Illinois, USA)
3:05 pm10 + 5 minDissection of Endothelial Junctional Dynamics Following Angiogenic Stimulation Through Application of an Engineered Optogenetic Biotin LigaseNicholas Leschinsky (University of Illinois, USA)


ibidi Industry Talk

3:25–4:00 pm

Industry Talk: Future Technologies and Applications for Cell Culture Under FlowNina Huber (ibidi GmbH, GER)


Poster Session

4:00–8:00 pm

Poster Session With Snacks and Drinks

July 25

Session 4:  Atherosclerosis

9:00–10:40 am

Chair: Julia Mack (University of California Los Angeles, USA)

9:00 am30 + 5 minKeynote: Endothelial Responses to Flow in AtherosclerosisPaul Evans (Queen Mary University of London, UK)
9:35 am30 + 5 minKeynote: Flow-Sensitive HEG1 Regulates Endothelial Function and Protects Against AtherosclerosisHanjoong Jo (Georgia Tech and Emory University, USA)
10:10 am10 + 5 minMicroRNA Deregulation as the Mechanism of Cadmium-Induced AtherosclerosisAbeer Mohamed
(University of Illinois, USA)
10:25 am10 + 5 minMechanosensitive Protein FHL2 Tunes Endothelial Flow ResponseShailaja Seetharaman
(The University of Chicago, USA)
10:40 am30 minCoffee break 


Session 5:  Renewal and Repair

11:10 am–1:05 pm

Chair: Yun Fang (The University of Chicago, USA)

11:10 am30 + 5 minKeynote: Mechanisms of Lymphatic Vessel Renewal and RepairTatiana Petrova (University of Lausanne, CH)
11:45 am30 + 5 minKeynote: Effect of Microfluidic on Endothelial Wound Regeneration During Streptococcal InfectionSimone Bergmann (Technical University Braunschweig, DE)
12:20 pm10 + 5 minElectro-Osmotic Flow Directs Cell Migration and Growth of Endothelial SproutsMark Messerli (South Dakota State University, USA)
12:35 pm10 + 5 minEnhancing the Targeting Efficacy of Endothelial Colony Forming Cells for Renal Regeneration via Kidney-Targeted Liposomal NanoparticlesBrenda Cruz-Gonzalez (University of Notre Dame, USA)
12:50 pm10 + 5 minShear-Sensitive Notch Activation via Extracellular Vesicles to Treat Vascular InjuriesPrerak Gupta (University of Illinois, USA)
1:05 pm55 minLunch break


Session 6:  Mechanotherapeutics, Cancer, Inflammation

2:00–3:35 pm

Chair: Luisa Iruela-Arispe (Northwestern University, USA)

2:00 pm30 + 5 minKeynote: Vascular Mechanobiology and Mechano-
therapeutics
Yun Fang (The University of Chicago, USA)
2:35 pm10 + 5 minDisturbed Flow Induces Reprogramming of Endothelial Cells
to Immune-Like and Foam Cells Under Hypercholesterolemia During Atherogenesis
Christian Park (Emory University & Georgia Tech, USA)
2:50 pm10 + 5 minMechano-Sensitive ER Protein TXNDC5 Promotes Neointimal Hyperplasia of Arterio-Venous Fistula Through Endothelial-Mesenchymal Transition and eNOS SuppressionChih-Fan Yeh (National Taiwan University Hospital, TW)
3:05 pm10 + 5 minThe Role of Endothelial Ackr1 in Breast Cancer MetastasisS. Tanner Roach (University of Illinois, USA)
3:20 pm10 + 5 minEndothelial Caveolin-1 and CXCL10 Promote Transcellular Migration of Autoreactive T Cells Across the Blood-Brain BarrierAli A. Almousawi (University of Illinois, USA)


Closing Remarks and Young Scientist Award

3:35–4:00 pm

Luisa Iruela-Arispe (Northwestern University, USA) / Roman Zantl (ibidi GmbH, GER)

3:35 pm10 minYoung Scientist Award
3:45 pm10 minClosing Remarks

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How to Get There

Meeting location: Simpson Querrey Building, Main Atrium, Ground Floor, 303 E Superior St, Chicago, IL 60611, USA

Public parking: Erie Ontario Self Park, 321 E Erie St, Chicago, IL 60611

Directions from parking: Exit the parking ramp on E Erie St and turn left (west). Walk to N Fairbanks Ct and turn right (north). In one block you will reach the Simpson Querrey Building, which is between E Huron St and E Superior St on N Fairbanks Ct. There are entrances to the building on both Huron (south entrance) and Erie (north entrance) and both entrances lead to the main atrium, where the conference will be held.

Additional parking: Northwestern Garage, 222 East Huron Street, Chicago, IL 60611

Date:July 24–25, 2024

Download Abstract Book

Location:logo_northwestern.png
Northwestern University, Chicago, IL, USA
Organizers:Luisa Arispe, Northwestern University, Chicago, IL, USA
Roman Zantl, ibidi, Munich, Germany
Contact:E-mail: flowmeeting2024@ibidi.com
Phone USA: (844) 276-6363
Phone International: +49 89 520 46 17-0