7^th ibiTea on May 9, 2023: This event took place in the past, the registration is closed.

Visualization and Quantification
of Endothelial Wound Healing Under Flow

Presented by Simone Bergmann, Technische Universität Braunschweig, Germany

Microscopic immunofluorescence images of primary human umbilical vein cells (HUVEC) under defined flow.
After 24 hours under a continuous flow of 10 dyn/cm², HUVEC are aligned in the direction of flow (white arrow) and migrated toward the defined cell-free gap previously created by the dual-chamber silicon inlets. Image magnification visualizes representative cells of the cell area within the white square. The actin cytoskeleton was detected with Alexa-488-conjugated Phalloidin, and the cell nuclei were stained with DAPI. Yellow arrows point to cell-cell-contacts. Images were obtained with CLSM (Leica Sp8) at 200 x magnification.

ibiTea Topic Outline

In our 7^th ibiTea, speaker Simone Bergmann will present her work on a systemic bacterial infection that often leads to inflammatory vascular damage and endothelial barrier leakage. The research group combined a wound healing approach with the ibidi Pump System in order to visualize and quantify endothelial regeneration under defined shear stress conditions after pneumococcal infection. Defined cell gaps were created using dual chamber silicon inlets (Culture-Inserts) to analyze cellular wound healing.

After flow cultivation, a specific immunofluorescent staining protocol enables microscopic analysis and provides new insights into cellular healing processes. This combined technical approach can be applied to a variety of pharmaco-medical, infection biological, and immunological questions.

Speaker

Simone Bergmann
Technische Universität Braunschweig, Germany

Simone Bergmann has been an associate professor and group leader at the Institute of Microbiology at the Technische Universität Braunschweig since 2010. Her research focuses on the study of host-pathogen interactions, and is specialized in pneumococcal and streptococcal infections. Several cell culture infection systems have been established in her laboratory in recent years, and her expertise has expanded to include the interaction between streptococci and primary endothelial cells.