Immunofluorescence Assays

Immunofluorescence (IF) Assays

The Principle of Immunofluorescence Assays

A Typical Workflow

Immunofluorescence With the ibidi Chambers

Experimental Examples

ibidi Products for Immunofluorescence, Immunocytochemistry, and Immunohistochemistry

µ-Slide VI 0.4

µ-Slide VI 0.5 Glass Bottom

µ-Slide I 0.4 Luer

µ-Slide 8 Well high

3 Well Chamber, removable

8 Well Chamber, removable

12 Well Chamber, removable

sticky-Slide Tissue

µ-Dishes

ibidi Mounting Medium

ibidi Mounting Medium with DAPI

Find more products for this application here.

Application Guide

Download the "Immunofluorescence" Application Guide as a PDF.

Selected Publications

K.G. Zyner, A. Simeone, S.M. Flynn, et al. G-quadruplex DNA structures in human stem cells and differentiation. Nat Commun, 2022, 10.1038/s41467-021-27719-1
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C. Xu, et al. NPTX2 promotes colorectal cancer growth and liver metastasis by the activation of the canonical Wnt/beta-catenin pathway via FZD6. Cell Death & Disease, 2019, 10.1038/s41419-019-1467-7
Read article

Kobayashi, T., et al. Principles of early human development and germ cell program from conserved model systems. Nature, 2017, 10.1038/nature22812
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H. Tada et al. Porphyromonas gingivalis Gingipain-Dependently Enhances IL-33 Production in Human Gingival Epithelial Cells. PloS one, 2016, 10.1371/journal.pone.0152794
Read article

Find more publications in the ibidi Reference Database.

AN 15: Fluorescence Staining using a µ-Slide y-shaped (PDF)

AN 16: Immunofluorescence Staining Using the µ-Slide 8 Well high (PDF)

AN 33: Live / Dead Staining with FDA and PI (PDF)

AN 44: Immunofluorescence Staining of HUVEC in 3D in the μ-Slide Chemotaxis (PDF)

AN 58: Immunofluorescence Staining Using the μ-Slide 18 Well (PDF)

AN 61: Immunofluorescence Staining of HUVECs on a Gel Matrix Using the µ-Slide I Luer 3D (PDF)

Videos

MV 18: Immunofluorescence Using the µ-Slide VI

MV 43: Handling the 8 Well Chamber, removable

ibidi Blog Article

Are you struggling to get optimal IF results? Shuntaro Yamada from the University of Bergen explains "5 Crucial Steps for Obtaining a Great Immunofluorescent Cell Image".

User Protocols

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UP 10: Immunofluorescent Staining of Adherent Cells in the ibidi µ-Slide 8 Well (PDF)

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UP 13: Protocol for Immunofluorescent Staining of iPS Cell-Derived Dopaminergic Neurons in the ibidi μ-Plate 96 Well Black (PDF)

Webinars

How to Setup a Successful
Immunofluorescence Experiment

Successful Immunofluorescence Image Acquisition and Quantification

Successful Immunofluorescence Image Acquisition and Quantification