Visualization of Endothelial Cell Junctions

Immunofluorescence staining of primary mouse brain microvascular endothelial cells (pMBMECs), cultured in the ibidi 12 Well Chamber, removable. Red color delineates the endothelial cell junctions labeled for zonula occludens (ZO)-1, highlighting their matured state. Blue color shows the nuclei stained with DAPI. The image was acquired with a 20x objective on a Nikon Eclipse microscope.

Data by Sidar Aydin, Britta Engelhardt, Ruth Lyck, Theodor Kocher Institute, Bern, Switzerland.


ibidi product used:


12 Well Chamber, removable

IF_pMBMECs.jpg

Immunostaining of Rat Dorsal Root Ganglionic Cells and Schwann Cells

Rat dorsal root ganglionic cells and Schwann cells cultured in an ibidi μ-Slide 8 Well and stained for neurofilament (green), NGFR (magenta), and DAPI (white). The image was obtained with a LEICA SP8X laser scanning microscope.

Data by Tamara Weiss, Division of Plastic and Reconstructive Surgery, Medical University of Vienna, Austria.


ibidi product used:


μ-Slide 8 Well

IF_dorsal_root_ganglion_schwann_cells.jp

Rat Hippocampal Neuron and Astrocyte Staining

Confocal microscopy of rat hippocampal neurons plated over astrocytes, cultured on ibidi µ-Plate 24 Well. Red is Synapsin 1, a pre-synaptic marker, white is neuron-specific Tubulin Beta-III, and blue is DAPI, a nuclear counterstain.

Data by Daniel Hoeppner, Lieber Institute for Brain Development. Baltimore, MD, USA.


ibidi product used:


µ-Plate 24 Well

IF_neurons_astrocytes.jpg

Adherens Junctions and Actin Cytoskeleton of HUVECs Under Flow

Human umbilical vein endothelial cells (HUVECs) were cultured under flow conditions in the ibidi μ-Slide I 0.4 Luer. Red: actin cytoskeleton (Cy5-conjugated antibody).
Green: adherens junctions, marked by VE-cadherin (Alexa 488-conjugated antibody). Blue: nuclear counterstaining (DAPI).

Data by S. Zahler, Munich, Germany.


ibidi product used:


μ-Slide I 0.4 Luer

IF_HUVECs_under_flow.jpg

Mitochondria Staining of MDCK cells

Madin-Darby canine kidney (MDCK) cells were cultured in the ibidi μ-Slide VI 0.4.
Green: F-actin cytoskeleton (Alexa488-Phalloidin). Red: mitochondria (MitoTracker). Blue: nuclear counterstaining (DAPI). Widefield fluorescence using 100x objective lens with oil immersion.


ibidi product used:


μ-Slide VI 0.4

IF_mitochondria_staining.jpg

Focal Adhesions of Differentiated Mouse Fibroblasts on an Elastic Surface

Differentiated mouse fibroblasts were cultured on an elastic surface using the µ-Dish 35 mm, high ESS (28 kPa). Green: focal adhesions, marked by zyxin (Alexa 488-conjugated antibody). Red: alpha-smooth muscle actin (Cy5-conjugated antibody).

Data by R. Merkel, Jülich, Germany


ibidi product used:


µ-Dish 35 mm, high ESS

D_81191_dish_35mmhigh_ESS_image1.jpg

Read on and learn more about the Principle of Immunofluorescence Assays, or see a typical Workflow of an Immunofluorescence Staining.