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12 Well Chamber, removable

12 Well Chamber, removable View larger

Chamber slide with a removable 12 well silicone chamber for cell culture and immunofluorescence staining

  • The ideal chambered slide for cost-effective immunofluorescence assays and long-term sample storage
  • Suitable for use with upright and inverted microscopes
  • Small staining volume for reduction of required medium, cells, and supplements

More details

microscopy glass slide, sterilized
15

Product Details

Applications:

  • Immunofluorescence assays using standard coverslip techniques
  • Long-term sample storage
  • 12 fold sample preparation of adherent cells and tissue samples

Specifications:

Number of wells 12
Dimensions of wells (w x l x h) in mm 7.5 x 7.5 x 8
Volume per well 250 µl
Growth area per well 0.56 cm²
Coating area per well 1.90 cm²
Material Biocompatible silicone
Suitable coverslip size 24 mm x 60 mm
Bottom Glass slide (26 x 76 x 1 mm)

Technical Features:

  • Self-adhesive silicone gasket mounted on a standard glass slide
  • Biocompatible silicone material
  • Non-fluorescent glass slide with frosted ends for easy handling and labeling
  • Suitable for use with upright and inverted microscopes, after staining and mounting with a coverslip
  • Compatible with all common mounting media
  • May require coating to promote cell attachment
  • Optional: The gasket may be transferred to any flat clean surface for cell cultivation

Define and print your
experimental setup

Standard Procedure 3 Well | 8 Well | 12 Well Chamber, removable:

Staining Protocol:

Supporting Material

User Comments

Milena Leseva, The Stephan Angeloff Institute of Microbiology, Sofia, Bulgaria

"The 12 Well and 8 Well Chambers, removable worked very well. We used them for fluorescence staining for H4K16ac in non-adherent, neutrophil cells. We treated the cells with different compounds that are expected to change this modification.

Overall, we are extremely happy with how easy it is to use the cell culture chambers, especially when you have cells with different treatments. We liked the fact that the chamber format minimizes the volume of antibody that we required in order to get a good signal."

Milena Leseva
The Stephan Angeloff Institute of Microbiology
Sofia
Bulgaria
http://microbio.bas.bg/wordpress/index.php/en/

Max-Joseph Kraus, Praxis Geiselgasteig, Grünwald, Germany

"I really love these slides! They are the best that happened to my research workflow. I mainly use the removable 12-well chambers for the high resolution dark field microscopy of platelets and they work excellent for me."

Max-Joseph Kraus
Praxis Geiselgasteig
Grünwald, Germany

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