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- Screening | High Throughput
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3 Well Chamber, removable
Chamber slide with a removable 3 well silicone chamber for cell culture and immunofluorescence staining
- The ideal slide for cost-effective immunofluorescence assays and long-term sample storage
- Large growth area combined with a small staining volume (especially 3 Well Chamber, removable)
- Suitable for use with upright and inverted microscopes
Product Details
Applications:
- Immunofluorescence assays using standard coverslip techniques
- Long-term sample storage
- 3 fold sample preparation of adherent cells and tissue samples
Specifications:
| Number of wells | 3 |
| Dimensions of wells (w x l x h) in mm | 16.5 x 16.5 x 8.0 |
| Volume per well | 1100 µl (150 µl*) |
| Growth area per well | 1.66 cm² |
| Coating area per well | 3.73 cm² |
| Material | Biocompatible silicone |
| Suitable coverslip size | 24 mm x 60 mm |
| Bottom | Glass slide (26 x 76 x 1 mm) |
* Volume reduction for staining when using a 15 mm glass coverslip (Cat. No. 10815)
Technical Features:
- Self-adhesive silicone gasket mounted on a standard glass slide
- Biocompatible silicone material
- Non-fluorescent glass slide with frosted ends for easy handling and labeling
- Suitable for use with upright and inverted microscopes, after staining and mounting with a coverslip
- Staining volume reduction by glass coverslip
- Compatible with all common mounting media
- May require coating to promote cell attachment
- Optional: The gasket may be transferred to any flat clean surface for cell cultivation
Standard Procedure 3 Well | 8 Well | 12 Well Chamber, removable:
Low Volume Staining Procedure 3 Well Chamber, removable:
Staining Protocol:
Supporting Material
Instructions
Application Notes
Product Flyer
FAQ
- Which mounting medium is recommended for mounting glass slides and coverslips in the Immunofluorescence Chambers 3 Well | 8 Well | 12 Well Chamber, removable?
- Can the ibidi Mounting Medium be used with the Immunofluorescence Chambers 3 Well | 8 Well | 12 Well Chamber, removable?
- Is the silicon gasket of the Immunofluorescence Chambers 3 Well | 8 Well | 12 Well Chamber, removable reusable?
- Can I use the 3 Well Chamber, removable without the 15 mm glass coverslip?
- µ-Slides and µ-Dishes
- Which solvents and chemicals are compatible with the ibidi labware?
User Comments
Petra Weber, Aalen University, Optical Engineering, Aalen, Germany
"We have tested the Removable Chambers (3 Well and 8 Well) and were very happy. Due to our very sensitive TIRF microscopy with an upright microscope we need appropriate glass slides with removable chambers. We tested adherent HeLa cells to detect Förster Resonance Energy Transfer between EGFR-CFP and Grb2-YFP with Fluorescence Lifetime Imaging. In particular for the activation or inhibition of intracellular signaling pathways, we need the subdivisions on the object slide. It was very easy to remove the chambers. Thank you very much for the possibility to test the chambers for free. I am sure I will use them again."
Petra Weber
Aalen University, Optical Engineering
Aalen, Germany
https://www.hs-aalen.de/de
