Triple immunofluorescence of bovine endothelial cells Red: mitochondria, stained with MitoTracker™ Red CMXRos Green: F-actin, stained with Alexa Fluor™ 488 Phalloidin Blue: nuclei, stained with DAPI
Lid with Locking Feature for Minimized Evaporation
All ibidi µ-Dishes are equipped with the special lid locking feature. The locking position minimizes evaporation and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide, or oxygen) during cell culture is maintained thanks to the gas-permeable plastic material of the dish.
TIP: Use the locking feature only if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).
1 High resolution microscopy = high magnification (40x - 100x) and high resolution microscopy including widefield or confocal techniques (e.g., brightfield, DIC, fluorescence). The resolution is limited to ~200 nm by the diffraction limit. Does not include Super-Resolution. Resolution is described as a function to discriminate 2 dots from each other; it is dependent on wavelength and numerical aperture. It is physically limited by Abbe’s law which can only be overcome by Super-Resolution microscopy.
2 Super-Resolution microscopy = additional resolution by overcoming the diffraction limit with 63x - 100x oil immersion objectives. Super-Resolution provides a resolution below 200 nm, down to 5-20nm by computational (PALM, SIM, STORM, RESOLVED) and one optical Super-Resolution technology (STED). TIRF is not a Super-Resolution technology.
³ DIC Compatibility = The lids are not DIC compatible. Special glass DIC Lids for the µ-Dishes are available separately.
Anuradha Vajjala, Nanyang Technological University, Singapore Centre on Environmental Life Sciences Engineering, Singapore
"To study the ultra structure of Streptococcus pyogenes biofilm grown on mouse embryonic fibroblasts, I used the ibiTreat 35mm μ-Dish to optimize the right conditions of biofilm growth. Simply awesome! It has made life so much simpler for me. Tissue culture combined with microscopy is so much easier now. The dishes are very user-friendly too. I have used the ibidi products to get very good quality images. I am really happy with the products!"
Anuradha Vajjala Nanyang Technological University Singapore Centre on Environmental Life Sciences Engineering Singapore
Randy Van Der Ploeg, St. Boniface Research Centre, Winnipeg, Manitoba, Canada
“We are pleased with the ibiTreat 35 mm culture dishes. I have given some of your product to one of our grad students to try and he is amazed with the robust axonal outgrowth that he finds only after one day of seeding cells. I have tested other dishes from your competitors and I did not get the reproducibility in growth characteristics like the ibiTreat µ-Dish.
The 35 mm dish holds up wonderfully to automated scanning over a wide scan area (essentially 98% of the growth surface area) which demonstrates the optical clarity and focal stability of the surface over such a wide area (1296 scan fields per well). I look forward to using more of the ibidi product lines in the future.”
Randy Van Der Ploeg Division of Neurodegenerative Disorders St. Boniface Research Centre Winnipeg, Manitoba Canada
Matthew Taylor, PhD, Princeton University, Princeton, USA
"The 35 mm µ-Dish is invaluable for high resolution imaging performed in support of my work. The optical plastic is fantastic substrate for building compartmentalized neuronal cultures. Thank you for making great products.”
Matthew Taylor Post-doctoral Fellow Department of Molecular Biology Princeton University Princeton, NJ USA
„Actually, ibidi dishes have single-handedly revolutionized my science. The cells that I use (horizontal retinal cells from fish) are extremely intolerant to being plated on any form of glass, even if the glass is treated with several different compounds. Because of this issue, I was only able to perform fluorescent microscopy on upright confocal microscopes; they allowed us to plate our cells on plastic dishes and image from above. Now, with the ibidi dishes, we are able to use better quality inverted microscopes and higher powered objectives. Our results have proven strong, and we have mentioned the power of ibidi products in our most recent publication.
I am so happy that I learned of your great products through a colleague. Microscopy will never be the same for our laboratory. So far I have purchased 3 boxes of 35mm ibiTreat high dishes. Thank you very, very much.”
Jason Jacoby PhD Candidate Laboratory of Neuroscience University of Illinois at Chicago USA