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µ-Dish 35 mm, high

µ-Dish 35 mm, high_ia View larger

A 35 mm imaging dish with an ibidi Polymer Coverslip Bottom, used in high end microscopy and cell-based assays

  • High walls with a standard height for easy handling
  • Unique ibidi Polymer Coverslip combining
    • Excellent cell culture conditions; and
    • Supreme optical quality for high resolution microscopy
  • Ideal cell growth conditions provided by the ibiTreat surface
  • Lid with lock position, which minimizes evaporation
  • Suitable for DIC, when used with the special DIC Lid
  • Also available with a relocation grid

More details

60

Buy 4 - Get 1 Free

Quantity Discount
5 20%

Product Details

Applications:

Technical Features

  • Standard format dish with a 35 mm diameter
  • Made of biocompatible material using no glues, consequently no cells are harmed
  • Rim for easy opening
  • No autofluorescence
  • Compatible with all staining and fixation solvents


High walls

Specifications:

Ø µ-Dish 35 mm
Volume 2 ml
Growth area 3.5 cm2
Coating area using 400 µl 4.1 cm2
Ø observation area 21 mm
Height with / without lid 14/12 mm
Bottom: ibidi Polymer Coverslip



Immunofluorescence of mouse cells
Blue: nucleus (DAPI)
Green: tubulin (Alexa488)

Lid with Lock Position

The special lock feature in all ibidi µ-Dishes enables the user to minimize evaporation. The lock position provides excellent conditions for long term studies in non humidified environments. Gas exchange (carbon dioxide, oxygen) during cell culture is maintained thanks to the gas permeable plastic material of the dish.

TIP: Use the locking feature only if minimal evaporation is required, i.e., outside incubators, non humidified microscopy stages, etc.

ibidi µ-Dish Family: Versions and Variations

High walls:
Used for all standard applications

Low walls:
Provide greater cell access (useful for micromanipulation)

Detailed Comparison ibidi Dishes 35 mm

µ-Dish 35 mm, high
(Polymer Coverslip,
ibiTreat / Uncoated)

81156, 81151
Glass Bottom Dish 35 mm

81218-200
81218-800
Bottom Thickness
180 µm (+10/-5 µm)
170 µm (+/-5 µm)
170 µm (+20/-10 µm)
Bottom Material
#1.5 Polymer coverslip
(ibidi)
#1.5H glass coverslip
(D 263 M Schott high precision glass)
#1.5 glass coverslip
(D 263 M Schott high precision glass)
Lid
Lid lock for minimal evaporation
and safe handling
Lid lock for minimal evaporation
and safe handling
Standard
Surfaces
ibiTreat (tissue culture treated),
Uncoated (hydrophobic)
Uncoated glass
Uncoated glass
Gas permeable
Yes
No
No
Packaging
Sterile, single-packed
Sterile, single-packed
Sterile, packed at 10 pcs.
Quantity
60 pcs./box
60 pcs./box
200/800 pcs./box
Free Sample
Yes
Yes
Yes

Microscopy Applications:

High resolution microscopy1
Confocal
+
+
+
Brightfield, Phase Contrast, DIC
+
+
+
Widefield Fluorescence
+
+
+
TIRF
-
+
-
Super-Resolution microscopy2
STED, STORM, etc.
-
+
-

Optical Properties:

Refractive Index
(nD 589 nm)
1.52
1.52
1.52
Abbe number
55
56
56
Autofluorescence
Low
Low
Low
Transmission
Very high (even ultraviolet)
High (ultraviolet restrictions)
High (ultraviolet restrictions)
Birefringence (DIC)

Note:

1 High resolution microscopy = high magnification (40x - 100x) and high resolution microscopy including widefield or confocal techniques (e.g., brightfield, DIC, fluorescence). The resolution is limited to ~200 nm by the diffraction limit. Does not include Super-Resolution.
Resolution is described as a function to discriminate 2 dots from each other; it is dependent on wavelength and numerical aperture. It is physically limited by Abbe’s law which can only be overcome by Super-Resolution microscopy.

2 Super-Resolution microscopy = additional resolution by overcoming the diffraction limit with 63x - 100x oil immersion objectives. Super-Resolution provides a resolution below 200 nm, down to 5-20nm by computational (PALM, SIM, STORM, RESOLVED) and one optical Super-Resolution technology (STED). TIRF is not a Super-Resolution technology.

³ DIC Compatibility = The lids are not DIC compatible. Special glass DIC Lids for the µ-Dishes are available separately.

Supporting Material

User Comments

Anuradha Vajjala, Nanyang Technological University, Singapore Centre on Environmental Life Sciences Engineering, Singapore

"To study the ultra structure of Streptococcus pyogenes biofilm grown on mouse embryonic fibroblasts, I used the ibiTreat 35mm μ-Dish to optimize the right conditions of biofilm growth. Simply awesome! It has made life so much simpler for me. Tissue culture combined with microscopy is so much easier now. The dishes are very user-friendly too. I have used the ibidi products to get very good quality images. I am really happy with the products!"

Anuradha Vajjala
Nanyang Technological University
Singapore
Centre on Environmental Life Sciences Engineering
Singapore

http://www.scelse.sg/

Randy Van Der Ploeg, St. Boniface Research Centre, Winnipeg, Manitoba, Canada

“We are pleased with the ibiTreat 35 mm culture dishes. I have given some of your product to one of our grad students to try and he is amazed with the robust axonal outgrowth that he finds only after one day of seeding cells.  I have tested other dishes from your competitors and I did not get the reproducibility in growth characteristics like the ibiTreat µ-Dish.

The 35 mm dish holds up wonderfully to automated scanning over a wide scan area (essentially 98% of the growth surface area) which demonstrates the optical clarity and focal stability of the surface over such a wide area (1296 scan fields per well). I look forward to using more of the ibidi product lines in the future.”    

Randy Van Der Ploeg
Division of Neurodegenerative Disorders
St. Boniface Research Centre
Winnipeg, Manitoba
Canada

http://www.sbrc.ca/dnd/

Matthew Taylor, PhD, Princeton University, Princeton, USA

"The 35 mm µ-Dish is invaluable for high resolution imaging performed in support of my work. The optical plastic is fantastic substrate for building compartmentalized neuronal cultures. Thank you for making great products.”

Matthew Taylor
Post-doctoral Fellow
Department of Molecular Biology
Princeton
University
Princeton
, NJ
USA
  

http://molbio.princeton.edu/

Jason Jacoby, University of Illinois, USA

„Actually, ibidi dishes have single-handedly revolutionized my science. The cells that I use (horizontal retinal cells from fish) are extremely intolerant to being plated on any form of glass, even if the glass is treated with several different compounds. Because of this issue, I was only able to perform fluorescent microscopy on upright confocal microscopes; they allowed us to plate our cells on plastic dishes and image from above. Now, with the ibidi dishes, we are able to use better quality inverted microscopes and higher powered objectives. Our results have proven strong, and we have mentioned the power of ibidi products in our most recent publication.

I am so happy that I learned of your great products through a colleague. Microscopy will never be the same for our laboratory. So far I have purchased 3 boxes of 35mm ibiTreat high dishes. Thank you very, very much.”

Jason Jacoby PhD Candidate
Laboratory of Neuroscience

University of Illinois at Chicago
USA

http://www.uic.edu/las/LIN

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