µ-Dish 35 mm, high

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µ-Dish 35 mm, high

A 35 mm imaging dish with an ibidi Polymer Coverslip Bottom, used in high end microscopy and cell-based assays

High walls with a standard height for easy handling

Unique ibidi Polymer Coverslip combining
Excellent cell culture conditions; and

Supreme optical quality for high resolution microscopy

Ideal cell growth conditions provided by the ibiTreat surface

Lid with lock position, which minimizes evaporation

Suitable for DIC, when used with the special DIC Lid

Also available with a grid for cell location and counting
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A 35 mm imaging dish with an ibidi Polymer Coverslip Bottom, used in high end microscopy and cell-based assays

High walls with a standard height for easy handling
Unique ibidi Polymer Coverslip combining
- Excellent cell culture conditions; and
- Supreme optical quality for high resolution microscopy
Ideal cell growth conditions provided by the ibiTreat surface
Lid with lock position, which minimizes evaporation
Suitable for DIC, when used with the special DIC Lid
Also available with a grid for cell location and counting

More details

Surface Modification

Pcs./Box

Product Details

Applications

Cell culture and high resolution fluorescence microscopy
Immunofluorescence staining
Live cell imaging
Transfection
Suitable for DIC, when used with the special DIC Lid

Specifications

Ø µ-Dish	35 mm
Volume	2 ml
Growth area	3.5 cm²
Coating area using 400 µl	4.1 cm²
Ø observation area	21 mm
Height with / without lid	14/12 mm
Bottom: ibidi Polymer Coverslip

Technical Features

Standard format dish with a 35 mm diameter
Made of biocompatible material using no glues, consequently no cells are harmed
Rim for easy opening
No autofluorescence
Compatible with all staining and fixation solvents

High walls

Experimental Example

Triple immunofluorescence of bovine endothelial cells
Red: mitochondria, stained with MitoTracker™ Red CMXRos;
Green: F-actin, stained with Alexa Fluor™ 488 Phalloidin;
Blue: nuclei, stained with DAPI.

Lid with Locking Feature for Minimized Evaporation

All ibidi µ-Dishes are equipped with the special lid locking feature. The locking position minimizes evaporation and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide, or oxygen) during cell culture is maintained thanks to the gas-permeable plastic material of the dish.

TIP: Use the locking feature only if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).

ibidi µ-Dish Family: Versions and Variations

µ-Dish^{35 mm, high}

µ-Dish^{35 mm, low}

µ-Dish^{50 mm, low}

High walls:
Used for all standard applications

Low walls:
Provide greater cell access (useful for micromanipulation)

Detailed Comparison ibidi Dishes^{35 mm}

	µ-Dish^{35 mm, high} (Polymer Coverslip, ibiTreat / Uncoated) 81156, 81151	µ-Dish^{35 mm, high} Glass Bottom 81158	Glass Bottom Dish^{35 mm} 81218-200 81218-800
Bottom Thickness	180 µm (+10/-5 µm)	170 µm (+/-5 µm)	170 µm (+20/-10 µm)
Bottom Material	#1.5 Polymer coverslip (ibidi)	#1.5H glass coverslip (D 263 M Schott high precision glass)	#1.5 glass coverslip (D 263 M Schott high precision glass)
Lid	Lid lock for minimal evaporation and safe handling	Lid lock for minimal evaporation and safe handling	Standard
Surfaces	ibiTreat (tissue culture treated), Uncoated (hydrophobic)	Uncoated glass	Uncoated glass
Gas permeable	Yes	No	No
Packaging	Sterile, single-packed	Sterile, single-packed	Sterile, packed at 10 pcs.
Quantity	60 pcs./box	60 pcs./box	200/800 pcs./box
Free Sample	Yes	Yes	Yes

Microscopy Applications:

High resolution microscopy¹
Confocal	+	+	+
Brightfield, Phase Contrast, DIC	+	+	+
Widefield Fluorescence	+	+	+
TIRF	-	+	-
Super-Resolution microscopy²
STED, STORM, etc.	-	+	-

Optical Properties:

Refractive Index (n_D 589 nm)	1.52	1.52	1.52
Abbe number	55	56	56
Autofluorescence	Low	Low	Low
Transmission	Very high (even ultraviolet)	High (ultraviolet restrictions)	High (ultraviolet restrictions)
Birefringence (DIC)	Low (DIC compatible³)	Low (DIC compatible³)	Low (DIC compatible³)

Note:

¹ High resolution microscopy = high magnification (40x - 100x) and high resolution microscopy including widefield or confocal techniques (e.g., brightfield, DIC, fluorescence). The resolution is limited to ~200 nm by the diffraction limit. Does not include Super-Resolution.
Resolution is described as a function to discriminate 2 dots from each other; it is dependent on wavelength and numerical aperture. It is physically limited by Abbe’s law which can only be overcome by Super-Resolution microscopy.

² Super-Resolution microscopy = additional resolution by overcoming the diffraction limit with 63x - 100x oil immersion objectives. Super-Resolution provides a resolution below 200 nm, down to 5-20nm by computational (PALM, SIM, STORM, RESOLVED) and one optical Super-Resolution technology (STED). TIRF is not a Super-Resolution technology.

³ DIC Compatibility = The lids are not DIC compatible. Special glass DIC Lids for the µ-Dishes are available separately.

Supporting Material

Instructions

Instructions µ-Dish^{35mm, high} (PDF)

Application Notes

Product Flyer

FAQ

User Comments

Anuradha Vajjala, Nanyang Technological University, Singapore Centre on Environmental Life Sciences Engineering, Singapore

"To study the ultra structure of Streptococcus pyogenes biofilm grown on mouse embryonic fibroblasts, I used the ibiTreat 35mm μ-Dish to optimize the right conditions of biofilm growth. Simply awesome! It has made life so much simpler for me. Tissue culture combined with microscopy is so much easier now. The dishes are very user-friendly too. I have used the ibidi products to get very good quality images. I am really happy with the products!"

Anuradha Vajjala
Nanyang Technological University
Singapore Centre on Environmental Life Sciences Engineering
Singapore

http://www.scelse.sg/

Randy Van Der Ploeg, St. Boniface Research Centre, Winnipeg, Manitoba, Canada

“We are pleased with the ibiTreat 35 mm culture dishes. I have given some of your product to one of our grad students to try and he is amazed with the robust axonal outgrowth that he finds only after one day of seeding cells. I have tested other dishes from your competitors and I did not get the reproducibility in growth characteristics like the ibiTreat µ-Dish.

The 35 mm dish holds up wonderfully to automated scanning over a wide scan area (essentially 98% of the growth surface area) which demonstrates the optical clarity and focal stability of the surface over such a wide area (1296 scan fields per well). I look forward to using more of the ibidi product lines in the future.”

Randy Van Der Ploeg
Division of Neurodegenerative Disorders
St. Boniface Research Centre
Winnipeg, Manitoba
Canada

http://www.sbrc.ca/dnd/

Matthew Taylor, PhD, Princeton University, Princeton, USA

"The 35 mm µ-Dish is invaluable for high resolution imaging performed in support of my work. The optical plastic is fantastic substrate for building compartmentalized neuronal cultures. Thank you for making great products.”

Matthew Taylor
Post-doctoral Fellow
Department of Molecular Biology
Princeton University
Princeton, NJ
USA

http://molbio.princeton.edu/

Jason Jacoby, University of Illinois, USA

„Actually, ibidi dishes have single-handedly revolutionized my science. The cells that I use (horizontal retinal cells from fish) are extremely intolerant to being plated on any form of glass, even if the glass is treated with several different compounds. Because of this issue, I was only able to perform fluorescent microscopy on upright confocal microscopes; they allowed us to plate our cells on plastic dishes and image from above. Now, with the ibidi dishes, we are able to use better quality inverted microscopes and higher powered objectives. Our results have proven strong, and we have mentioned the power of ibidi products in our most recent publication.

I am so happy that I learned of your great products through a colleague. Microscopy will never be the same for our laboratory. So far I have purchased 3 boxes of 35mm ibiTreat high dishes. Thank you very, very much.”

Jason Jacoby PhD Candidate
Laboratory of Neuroscience
University of Illinois at Chicago
USA

http://www.uic.edu/las/LIN