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micro-Insert 4 Well FulTrac in µ-Dish 35 mm, high

micro-Insert 4 Well FulTrac in µ-Dish 35 mm, high View larger

A 4-well silicone insert used for tracing individual cells (adherent and suspension)

  • Allows for long-term microscopy assays of single cells
  • Cultivation of small numbers of cells without evaporation risks
  • Conical wells for superb optical quality
  • Complete coverage of the well using a 20x objective
  • Individual inserts for single experiments
  • Ideal cell growth on ibiTreat surface

More details

ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
30
in µ-Dish 35 mm, high

Buy 4 - Get 1 Free

Quantity Discount
5 20%

Product Details

Applications:

Technical Features:

  • Four small Ø 0.4 mm microscopy wells
  • Suspension cells cannot escape the observation field
  • Adhesive underside
  • Transferable to any flat, clean surface
  • No material is left behind after the insert is removed
  • Includes a rim for easy handling of the insert with tweezers

Specifications:

Number of wells 4
Dimensions of wells in mm Ø 0.4
Diameter of the complete insert 12 mm
Height of the complete insert 4.3 mm
Volume per well 10 µl
Growth area per well 0.0012 cm2
Coating area per well 0.188 cm2
Material Biocompatible silicone
Bottom No bottom – sticky underside

Cultivation of IVF mouse eggs in the micro-Insert 4 Well FulTrac (prototype; total imaging duration: 12d 17h 55m)
Courtesy of Dr. Asako Sakaue-Sawano and Dr. Atsushi Miyawaki, RIKEN, Japan
(Find more detailed information here.)

Product Selection Guide

micro-Insert
4 Well FulTrac
  • Single cells
  • 20x Objective lens for full well
    coverage
  • High resolution fluorescence
    microscopy
micro-Insert
4 Well
  • Few cells
  • 4x Objective lens for full well
    coverage
  • High resolution fluorescence
    microscopy

Cross Section

Ideal Coverage Using Digital Imaging with CCD Cameras

Possible Filling Procedures:

Minor Well Filling

  • Minimal volume
  • Individual wells

Whole Insert Filling

  • Moderate volume
  • Wells connected by medium

Whole µ-Dish Filling

  • Maximum volume
  • Maximum experimental duration

Evaporation Barrier

More Information

The micro-Insert 4 Well FulTrac was developed in cooperation with the RIKEN Brain Science Institute, Japan.
Reference Publication:
Sakaue-Sawano A, Hoshida T, Yo M, Takahashi R, Ohtawa K, Arai T, Takahashi E, Noda S, Miyoshi H and Miyawaki A. Visualizing developmentally programmed endoreplication in mammals using ubiquitin oscillators. The Company of Biologists Ltd | Development (2013) 140, 4624-4632
http://dev.biologists.org/content/develop/140/22/4624.full.pdf

Supporting Material

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