micro-Insert 4 Well FulTrac in µ-Dish 35 mm, high

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micro-Insert 4 Well FulTrac in µ-Dish 35 mm, high

A 4-well silicone insert used for tracing individual cells (adherent and suspension)

Allows for long-term microscopy assays of single cells

Cultivation of small numbers of cells without evaporation risks

Conical wells for superb optical quality

Complete coverage of the well using a 20x objective

Individual inserts for single experiments

Ideal cell growth on ibiTreat surface
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A 4-well silicone insert used for tracing individual cells (adherent and suspension)

Allows for long-term microscopy assays of single cells
Cultivation of small numbers of cells without evaporation risks
Conical wells for superb optical quality
Complete coverage of the well using a 20x objective
Individual inserts for single experiments
Ideal cell growth on ibiTreat surface

More details

Surface Modification

ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized

Pcs./Box

Product Variation

in µ-Dish 35 mm, high

Product Details

Applications:

Live cell imaging of adherent and suspension cells
Immunofluorescence assays
Performing long-term microscopy assays lasting up to several weeks
Cell tracking of single cells over time
Cell differentiation (e.g., single stem cells)

Specifications:

Number of wells	4
Dimensions of wells in mm	Ø 0.4
Diameter of the complete insert	12 mm
Height of the complete insert	4.3 mm
Volume per well	10 µl
Growth area per well	0.0012 cm²
Coating area per well	0.188 cm²
Material	Biocompatible silicone
Bottom	No bottom – sticky underside

Technical Features:

Four small Ø 0.4 mm microscopy wells
Suspension cells cannot escape the observation field
Adhesive underside
Transferable to any flat, clean surface
No material is left behind after the insert is removed
Includes a rim for easy handling of the insert with tweezers

Experimental Example

Cultivation of IVF mouse eggs in the micro-Insert 4 Well FulTrac (prototype; total imaging duration: 12d 17h 55m)
Courtesy of Dr. Asako Sakaue-Sawano and Dr. Atsushi Miyawaki, RIKEN, Japan
(Find more detailed information here.)

Product Selection Guide

micro-Insert
4 Well FulTrac

Single cells
20x Objective lens for full well
coverage
High resolution fluorescence
microscopy

micro-Insert
4 Well

Few cells
4x Objective lens for full well
coverage
High resolution fluorescence
microscopy

Cross Section

Ideal Coverage Using Digital Imaging with CCD Cameras

Possible Filling Procedures:

Minor Well Filling

Minimal volume
Individual wells

Whole Insert Filling

Moderate volume
Wells connected by medium

Whole µ-Dish Filling

Maximum volume
Maximum experimental duration

Evaporation Barrier

More Information

The micro-Insert 4 Well FulTrac was developed in cooperation with the RIKEN Brain Science Institute, Japan.
Reference Publication:
Sakaue-Sawano A, Hoshida T, Yo M, Takahashi R, Ohtawa K, Arai T, Takahashi E, Noda S, Miyoshi H and Miyawaki A. Visualizing developmentally programmed endoreplication in mammals using ubiquitin oscillators. The Company of Biologists Ltd | Development (2013) 140, 4624-4632
http://dev.biologists.org/content/develop/140/22/4624.full.pdf