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Application Notes in Numerical Order

AN 01: Gradients Inside µ-Slide I (PDF)
Establishing a concentration profile

AN 02: Fluorescence Staining using a µ-Slide I (PDF)
Examples describing how to do immunofluorescence stainings using µ-Slides

AN 03: Growing Cells in µ-Channels (PDF)
Growing cells inside a µ-Slide VI 0.4, and a comparison between channels and open wells

AN 04: Lipid Monolayer (PDF)
Description of the uncomplicated and fast preparation of a lipid monolayer on uncoated slides

AN 05: Tube Formation in µ-Plate Angiogenesis 96 well (PDF)
Handling protocol for tube formation assays using a multi-channel pipette and the µ-Plate Angiogenesis 96 well

AN 06: Trypsination in µ-Channels (PDF)
Removing adherently grown cells from a µ-channel after cultivation

AN 07: Gene Transfection (PDF)
Example showing how protocols for gene transfection can easily be adapted to the work in cell culture channels

AN 08: Cell Culture Coating (PDF)
Making your own coating on µ-slides

AN 09: Fluorescence Staining using a µ-Slide VI (PDF)
Examples describing how to do immunofluorescence stainings using µ-Slides

AN 10: Co-Cultivation (PDF)
Application note for co-cultivation of two different cell types in µ-Slide 2 Well Co-Culture

AN 11: Shear Stress and Shear Rates (PDF)
Detailed information on shear stress/shear rates and flow rates in our channel slides

AN 12: Avoiding Evaporation (PDF)
Decreasing evaporation during cell cultivation by using the ibidi humidifying chamber Olaf and µ-Slides

AN 13: HUVECs Under Perfusion (PDF)
Setting up a flow experiment using μ-Slide I and HUVECs

AN 15: Fluorescence Staining using a µ-Slide y-shaped (PDF)
Examples describing how to do immunofluorescence stainings using µ-Slides

AN 16: Fluorescence Staining using a µ-Slide 8 well (PDF)
Examples describing how to do immunofluorescence stainings using µ-Slides

AN 17: Chemotaxis 2D and 3D (PDF)
General protocol for 2D and 3D gel assays with µ-Slide Chemotaxis

AN 18: Shear Stress and Shear Rates in µ-Slide µ-Shaped (PDF)
Detailed information on shear stress/shear rates and flow rates in our µ-Slide y-shaped

AN 19: Tube Formation (PDF)
Setting up a tube formation assay with µ-Slide Angiogenesis

AN 20: Cultivation of Macrophages (PDF)
Cultivation protocol for a murine macrophage cell line in µ-Slide VI0.4 and µ-Slide 8 well

AN 21: Wound Healing Assay (PDF)
Setting up a wound healing assay with the ibidi Culture-Insert in a µ-Dish 35 mm

AN 22: Pixel Size (PDF)
Measuring and calculating the pixel size of microscopic images

AN 23: 3D Chemotaxis Protocol for Non-Adherent Cells in a Gel Matrix (PDF)
Application Note providing a specific example protocol for chemotaxis of dendritic cells in a collagen gel

AN 24: Chemotaxis of HT-1080 Cells in 2D and 3D (PDF)
Detailed protocol of experimental parameters and example data of HT-1080 cells migrating in 2D and in 3D in collagen I gels

AN 25: Serial Connection of Flow Chambers (PDF)
Setup protocol for connecting several Luer-Slides to one Fluidic Unit

AN 26: Collagen I Gel for 3D Cell Culture (PDF)
Fabrication protocols for collagen I gel (bovine and rat tail) with different cell media

AN 28: Adenoviral Transduction of Human Cells (PDF)
Detailed protocol for handling recombinant adenoviruses, plus designing an approach for transducing human cells

AN 29: Transfer of mRNA into iPSC-derived Cardiomyocytes Using Fuse-It-mRNA (PDF)
Detailed instructions for the optimization of the Fuse-It-mRNA protocol for iPSC-derived Cardiomyocytes in the µ-Slide VI 0.4

AN 31: Serial Connection of µ-Slide VI 0.4 (PDF)
Protocol for connecting the six channels of µ-Slide VI 0.4 to one Fluidic Unit

AN 32: Generation of Spheroids (PDF)
Generation of spheroids using the liquid overlay technique

AN 33: Live / Dead Staining with FDA and PI (PDF)
Viability staining of adherent cells, single cells embedded in extracellular matrix, and cellular clusters

AN 34: Chemotaxis of HUVEC Cells in 2D and 3D (PDF)
Detailed protocol of experimental parameters and example data of HUVEC cells migrating in 2D and in 3D in collagen I gels.

AN 35: Chemotaxis Assay with Cells Producing a Chemoattractant (PDF)
A detailed protocol for using adherent cells as chemoattractant-producers in the large reservoirs of the µ-Slide Chemotaxis.

AN 36: Wound Healing Assay in µ-Plate 24 Well (PDF)
A handling protocol for wound healing assays: Screening substances for pro- or anti-migrational effects

AN 37: Image Shift Correction in Microscopic Time Lapse Sequences (PDF)
Instructions for correcting an externally generated shift of the sample in time lapse images (e.g. in chemotaxis experiments)

AN 38: Western Blot Analysis with Cell Samples Grown in Channel-µ-Slides (PDF)
Detailed protocol for performing cell lysis in channel-µ-Slides and subsequent SDS-Page with Western Blot Analysis

AN 39: Cell Proliferation Assay in µ-Slide Angiogenesis (PDF)
A Detailed Protocol for Analyzing the Cell Proliferation Using MDCK Cells for the Example

AN 40: Gene Expression Profiling with Cell Samples Grown in Channel-µ-Slides (PDF)
Detailed protocol for performing cell lysis in channel-µ-Slides and subsequent RNA-Isolation with RT-qPCR Analysis

AN 41: Lentiviral Transduction of Human Cells (PDF)
Detailed protocol for handling recombinant lentiviruses, plus designing an approach for transducing human cells

AN 43: Transfer of mRNA into L929 Cells using Fuse-It-mRNA (PDF)
Detailed protocol for handling recombinant lentiviruses, plus designing an approach for transducing human cells

AN 45: Mounting Medium Types (PDF)
A comparison of non-hardening and hardening mounting media

AN 47: Electrical Stimulation of Cardiomyocytes in µ-Slide I Luer Electrode (PDF)
Typical parameters for the stimulation of cardiomyocytes using the µ-Slide I Luer Electrode as well as a seeding protocol for the cells

AN 48: Shear Stress and Shear Rates in ECIS Flow Arrays (PDF)
Detailed information on shear stress, shear rate, and flow rate calculations in ECIS Flow Arrays

AN 49: Fluorescence Staining using a 12 Well Chamber, removable (PDF)
A handling protocol for immunofluorescence staining in the 12 Well Chamber, removable

AN 50: Fluorescence Staining using a 3 Well Chamber, removable (PDF)
A handling protocol for immunofluorescence staining in the 3 Well Chamber, removable, including instructions for using the volume-minimizing coverslip

AN 52: Transfer of R-Phycoerythrin into CHO-K1 cells using Fuse-It-P (PDF)
Detailed instructions for the transfer of the protein R-Phycoerythrin into CHO-K1 cells using Fuse-It-P in the µ-Dish 35 mm, high

AN 54: Knockdown of GFP-Expression—A Comparison of Fusion and Lipofection (PDF)
Instructions on the handling of Fuse-It-siRNA, as well as a comparison of various siRNA transfection alternatives

AN 55: siRNA Transfection Into Primary Neurons Using Fuse-It-siRNA (PDF)
Instructions for siRNA transfection into sensitive, primary neurons using Fuse-It-siRNA, and knockdown analysis using quantitative real time polymerase chain reaction (qRT-PCR).