Imaging Small Organisms and Embryos

ibidi labware with small wells is suitable for small organism imaging and embryo imaging using samples such as Xenopus eggs, Drosophila embryos, C. elegans, and zebrafish (Danio rerio). The optically clear coverslip bottoms support high-resolution fluorescence microscopy and live cell imaging of living organisms, as well as imaging of fixed samples.

Small organism and embryo imaging using fluorescence microscopy in ibidi labware

The µ-Slide 18 Well – Flat provides 18 flat wells for imaging multiple small organisms or embryos in parallel. Its flat design and small well volume make it particularly suitable for short-term microscopy assays and high-resolution imaging of fixed samples.

µ-Slide 18 Well – Flat for parallel microscopy of small organisms and embryos

The µ-Slide 15 Well 3D provides 15 independent wells with a well-in-a-well geometry. Small organisms can be immobilized in a gel matrix in the inner well while remaining accessible through the upper well. The surrounding medium reservoir helps reduce evaporation and supports longer live cell imaging experiments.

µ-Slide 15 Well 3D for gel-based immobilization and live imaging of small organisms

The micro-Insert 4 Well is a removable silicone insert with four small, conical wells for positioning and imaging small organisms or embryos. Its adhesive underside allows the insert to be placed on flat, clean surfaces.

micro-Insert 4 Well with four conical wells for small organism imaging

The µ-Slide III 3D Perfusion is suitable for immobilizing small organisms in the wells while applying gentle perfusion across the sample. Continuous medium flow supports nutrient and oxygen supply during long-term cultivation and imaging under perfusion.

µ-Slide III 3D Perfusion for long-term cultivation and imaging of small organisms under flow

ibidi Products for Small Organism and Embryo Imaging

Key Product Specifications

ProductVolume per WellWell Size
µ-Slide 18 Well – Flat30 µl5 mm diameter
µ-Slide 15 Well 3D10 µl inner well
50 µl upper well
4 mm diameter (inner well)
5 mm diameter (upper well)
micro-Insert 4 Well10 µl2.0 × 1.5 mm
µ-Slide III 3D Perfusion30 µl5.5 mm diameter

For immobilizing small organisms during microscopy, we recommend using a gel matrix, such as agarose, collagen, or Matrigel®.