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µ-Slide III 3D Perfusion
A three channel µ-Slide used for the cultivation and perfusion of 3D cell structures
- Ideal for the long-term cultivation of cells in 3D matrices with an optimal nutrients supply
- Easy handling: Samples can be placed into the open wells–perfusion can be applied after closing the wells
- Excellent optical-quality imaging chamber for high-end microscopy
Product Details
Applications
- Observation of single cells in 3D matrices or tissue samples (e.g., spheroids, small organoids, or organisms)
- Perfusion of samples
- Long-term cultivation of cells in 3D matrices
Specifications
| Outer dimensions | 25.5 mm x 75.5 mm |
| Number of wells | 6 |
| Volume of wells | 30 µl |
| Well diameter | 5.5 mm |
| Well height (without channel) | 1.2 mm |
| Well height (with channel) | 1.7 mm |
| Growth area per well | 25 mm2 |
| Number of channels | 3 |
| Total channel volume | 130 µl |
| Channel width | 1.0 mm |
| Adapters | Female Luer |
| Volume per reservoir | 60 µl |
| Coating area using 130 µl | 2.4 cm2 per channel |
| Top cover matches coverslip | No. 1.5 |
| Bottom | ibidi Polymer Coverslip |
Technical Features
- Open wells are filled and later closed for the perfusion of the sample
- Channels allow for easy fluid connection of the wells
- Luer adapters for easy pump connection (e.g., to the ibidi Pump System)
- Optimal nutrients supply into 3D structures
- Observe the sample through the polymer coverslip bottom using high resolution light microscopy
The Principle of the µ-Slide 3D Perfusion
The µ-Slide 3D Perfusion contains six wells, in which cells can be cultivated in 3D matrices and imaged using high-resolution microscopy. Two of the six wells, respectively, are connected by a channel. For optimal nutrition of the cells during long-term culture, the channels can be connected to a pump for perfusing the wells.
Application Examples Using the µ-Slide 3D Perfusion
3D Matrix Culture
Single cells can be cultured and imaged in a 3D matrix, e.g., a collagen gel.
Soft Matrix Culture
Adherent cells can be cultured and imaged on a soft matrix.
Culture Without Matrix
Adherent cells can be seesed directly on the substrate without any matrix.
Spheroid Culture
Spheroids can be cultured and imaged, e.g., in a gel sandwich.
Tissue Sample Culture
Tissue samples can be cultured and imaged, e.g., in a gel sandwich.
Standard Filling Procedure
