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µ-Slide III 3D Perfusion

µ-Slide III 3D Perfusion View larger

A three channel µ-Slide used for the cultivation and perfusion of 3D cell structures

  • Ideal for the long-term cultivation of cells in 3D matrices with an optimal nutrients supply
  • Easy handling: Samples can be placed into the open wells–perfusion can be applied after closing the wells
  • Excellent optical-quality imaging chamber for high-end microscopy

More details

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Product Details

Applications

  • Observation of single cells in 3D matrices or tissue samples (e.g., spheroids, small organoids, or organisms)
  • Perfusion of samples
  • Long-term cultivation of cells in 3D matrices

Specifications

Outer dimensions 25.5 mm x 75.5 mm
Number of wells 6
Volume of wells 30 µl
Well diameter 5.5 mm
Well height (without channel) 1.2 mm
Well height (with channel) 1.7 mm
Growth area per well 25 mm2
Number of channels 3
Total channel volume 130 µl
Channel width 1.0 mm
Adapters Female Luer
Volume per reservoir 60 µl
Coating area using 130 µl 2.4 cm2 per channel
Top cover matches coverslip No. 1.5
Bottom ibidi Polymer Coverslip

Technical Features

  • Open wells are filled and later closed for the perfusion of the sample
  • Channels allow for easy fluid connection of the wells
  • Luer adapters for easy pump connection (e.g., to the ibidi Pump System)
  • Optimal nutrients supply into 3D structures
  • Observe the sample through the polymer coverslip bottom using high resolution light microscopy

The Principle of the µ-Slide 3D Perfusion

The µ-Slide 3D Perfusion contains six wells, in which cells can be cultivated in 3D matrices and imaged using high-resolution microscopy. Two of the six wells, respectively, are connected by a channel. For optimal nutrition of the cells during long-term culture, the channels can be connected to a pump for perfusing the wells.

Application Examples Using the µ-Slide 3D Perfusion

3D Matrix Culture

Single cells can be cultured and imaged in a 3D matrix, e.g., a collagen gel.

Soft Matrix Culture

Adherent cells can be cultured and imaged on a soft matrix.

Culture Without Matrix

Adherent cells can be seesed directly on the substrate without any matrix.

Spheroid Culture

Spheroids can be cultured and imaged, e.g., in a gel sandwich.

Tissue Sample Culture

Tissue samples can be cultured and imaged, e.g., in a gel sandwich.

Standard Filling Procedure

Supporting Material

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