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µ-Slide VI 0.5 Glass Bottom

µ-Slide VI 0.5 Glass Bottom View larger

A 6 channel μ-Slide suitable for flow experiments and for immunofluorescence assays

  • All-in-one chamber that simplifies immunofluorescence protocols
  • Homogeneous cell distribution over the channel surface, regardless of handling practices
  • Cost-effective experiments with small numbers of cells and low volumes of reagents
  • Perfect cell imaging thanks to the low thickness variability of the coverslips’ glass (# 1.5H)

More details

#1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized
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Product Details

Applications

Specifications

Outer dimensions (w x l) 75.5 x 25.5 mm
Adapters Female Luer
Number of channels 6
Channel volume 40 µl
Channel height 0.54 mm
Channel length 17 mm
Channel width 3.8 mm
Volume per reservoir 60 µl
Growth area 0.6 cm² per channel
Coating area using 40 µl 1.2 cm² per channel
Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm


Define and print your experimental setup

Technical Features

  • 40 µl channel volume, which saves on reagent consumption
  • No. 1.5H High Precision D 236M borosilicate glass coverslip
  • Fully compatible with high resolution fluorescence microscopy
  • Easy connection to existing tubes and pumps via female Luer adapter
  • Defined shear stress and shear rate level
  • Also available as a µ-Slide VI 0.4 with a Polymer Coverslip Bottom

All-in-One-Chamber for Fast Immunofluorescence Protocols

ibidi Polymer Coverslip vs. ibidi Glass Bottom

ibidi Polymer Coverslip

ibidi Glass Bottom

Optical properties
Refractive index (nD 589 nm) 1.52 1.52
Abbe number 56 55
Thickness #1.5 (180 µm) #1.5H (170 µm)
Material Microscopy plastic D 263M Schott borosilicate glass
Autofluorescence Low Low
Transmission Very high (even ultraviolet) High (ultraviolet restrictions)
Birefringence (DIC) Low (DIC compatible*) Low (DIC compatible*)
Other aspects
Surface modifications ibiTreat – tissue culture treated
Uncoated – hydrophobic
Only glass
Protein coatings Compatible Compatible
Gas permeable Yes No
Material flexibility High Low
Breakable No Yes
Applications Fluorescence microscopy TIRF and single photon

* Special glass DIC Lids are available separately

Supporting Material

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