Cell Culture Under Flow

Cell Culture Under Flow

Cell Culture Under Flow:
An Overview

The Flow Assay Experiment:
Planning, Setup & Analysis

Cells Under Flow:
Experimental Examples

Experimental Workflow

Experimental workflow

ibidi Products for Cell Perfusion Assays

ibidi Pump System

µ-Slide I 0.4 Luer

µ-Slide VI 0.1

µ-Slide VI 0.4

µ-Slide y-shaped

µ-Slide III 3D Perfusion

µ-Slide I Luer 3D

µ-Slide Spheroid Perfusion

µ-Slide ibiPore SiN

Find more products for cell culture under flow and cell perfusion here.

Application Guide

Download the whole "Cell Culture Under Flow" Application Guide as a PDF here.

Scientific Posters

Cell Culture Under Flow: Enhancing Cellular Physiology and Function Through Dynamic Microenvironments (PDF)

Cell Culture Under Flow: Enhancing Cellular Physiology and Function Through Dynamic Microenvironments (PDF)

Presented at the International Cell Culture Under Flow Meeting 2024, Chicago, USA.

Perfused 3D Cell Culture Platform for Long-term Monitoring of Glucose and Lactate Metabolism in Tumor Spheroids (PDF)

Perfused 3D Cell Culture Platform for Long-term Monitoring of Glucose and Lactate Metabolism in Tumor Spheroids (PDF)

Presented at the SelectBio Conference Organoids and Spheroids Europe 2024, Rotterdam, The Netherlands.

Changes in cell-cell contacts of endothelial cells (HUVEC) under long term shear stress conditions (PDF)

Changes in cell-cell contacts of endothelial cells (HUVEC) under long term shear stress conditions (PDF)

Presented at the North American Vascular Biology Organization (NAVBO) Conference 2014, Monterey, California, USA.

Defining the Critical Shear Stress Range in Long Term HUVEC Cell Culture (PDF)

Defining the Critical Shear Stress Range in Long Term HUVEC Cell Culture (PDF)

Presented at the 17th Barrier and Transporter Meeting 2015, Bad Herrenalb, Germany.

Selected Publications

D.C. 't Hart, J. van der Vlag, T. Nijenhuis. Laminar flow substantially affects the morphology and functional phenotype of glomerular endothelial cells. PLoS One, 2021, 10.1371/journal.pone.0251129
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N.M. Wakida, G.M.S. Cruz, P. Pouladian, M.W. Berns, D. Preece. Fluid Shear Stress Enhances the Phagocytic Response of Astrocytes. Front Bioeng Biotechnol, 2020, 10.3389/fbioe.2020.596577.
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I. Xanthis, C. Souilhol, J. Serbanovic-Canic, et al. β1 integrin is a sensor of blood flow direction. J Cell Sci, 2019, 10.1242/jcs.229542
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J. Xu, J. Mathur, E. Vessières, et al. GPR68 Senses Flow and Is Essential for Vascular Physiology. Cell, 2018, 10.1016/j.cell.2018.03.076, 0092-8674
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T. Keeley, R. Siow, R. Jacob and G. Mann. A PP2A-mediated feedback mechanism controls Ca2+-dependent NO synthesis under physiological oxygen. The FASEB Journal, 2017, 10.1096/fj.201700211R
Read article

A. Sabine, E. Bovay, C.S. Demir, et al. FOXC2 and fluid shear stress stabilize postnatal lymphatic vasculature. The Journal of Clinical Investigation, 2015, 10.1172/JCI80454
Read article

Find more publications in the ibidi Reference Database.

Application Notes

AN 03: Cell Culture in ibidi Channel Slides (PDF)

AN 11: Shear Stress and Shear Rates (PDF)

AN 13: Endothelial Cells Under Perfusion (PDF)

AN 14: Live Cell Imaging Under Flow (PDF)

AN 18: Shear Stress and Shear Rates in µ-Slide µ-Shaped (PDF)

AN 25: Serial Connection of Flow Chambers (PDF)

AN 31: Serial Connection of µ-Slide VI 0.4 (PDF)

AN 59: HUVECs Cultivated on the µ-Slide Membrane ibiPore Flow Under Shear Stress (PDF)

AN 60: HUVECs Under Shear Stress on a Collagen Matrix in the μ-Slide I Luer 3D (PDF)

AN 63: Generation and Dynamic Culture of L929 Spheroids in the µ-Slide Spheroid Perfusion (PDF)

Webinars

Webinar_Flow_2020.jpg

Introduction to Cell Culture Under Flow, Shear Stress, and Flow Patterns

Webinar_Flow_Setup_Applications.jpg

Cell Culture Under Flow: Setup and Applications

Webinar_Flow_Applications.jpg

Applications of Cell Culture Under Flow: Endothelial Response to Shear Stress

Webinar_Flow_Applications.jpg

Advanced Cell Culture Under Flow – Dynamic 3D Applications


Webinar_Flow_Applications.jpg

Going With the Flow—The Tale of Von Willebrand Factor

Videos

MV 20: The ibidi Pump System

MV 21: Long-Term Cell Culture Under Perfusion

Z-Stack Through a Spheroid Before and After Clearing in the µ-Slide Spheroid Perfusion

MV 48: Filling of the µ-Slide I Luer width=

MV 48: Filling of the µ-Slide I Luer


MV 49: Medium Exchange in the µ-Slide I Luer

MV 49: Medium Exchange in the µ-Slide I Luer


MV 50: Filling of the µ-Slide VI 0.4

MV 50: Filling of the µ-Slide VI 0.4


MV 51: Filling of the µ-Slide VI 0.4

MV 51: Medium Exchange in the µ-Slide VI 0.4


MV 57: Handling the µ-Slide I Luer 3D

MV 57: Handling the µ-Slide I Luer 3D


MV 58: Handling the µ-Slide Spheroid Perfusion

MV 58: Handling the µ-Slide Spheroid Perfusion

User Protocols

UP 08: Protocol for an Adhesion Assay Using Cell Culture Under Unidirectional Flow (PDF)

UP 11: Spheroid Culture, Staining, and Clearing for 3D Imaging (PDF)