Experimental Workflow
Cell Seeding Into Channel Slides
Procedure
The cell suspension is filled into the channel slide. After cell attachment, the flow experiment can be started. ibidi provides channel slides with different channel heights and geometries, which are suitable for various experimental conditions. For details, please refer to the chapter "The Optimal Perfusion Set and Slide for Your Application".
ibidi Products for This Step
Flow Conditioning of Adherent Cells
Procedure
After connecting the channel slide to the ibidi Pump System, the flow can be applied. Under flow, the cell culture medium is continuously pumped through the channel slide. This results in shear stress that influences the cell behavior and physiology. The flow type, flow rate, and the assay duration depend on the experimental setup. Find more details about how to plan the optimal experimental setup here.
ibidi Products for This Step
Staining and Image Acquisition
Procedure
Using live cell microscopy, images or videos of the flow-conditioned cell layer can be directly acquired within the channel slide. Further, immunofluorescence stainings and subsequent imaging can be performed directly in the channel slide.
ibidi Products for This Step
Quantitative and Statistical Analysis
Procedure
After the flow experiment, the cells can be easily analyzed using, e.g., Western blot, qPCR, FACS, and more downstream methods.
Read on and learn more about how to choose the Optimal Setup for Your Flow Assay, or which Questions to Ask Before Starting an Experiment.