Immunofluorescence With the ibidi Chambers

Chamber Slides

In the ibidi Chamber Slides, a silicone gasket with separated chambers is mounted on a standard glass slide. The ibidi Chamber Slides for immunofluorescence are ideal for the long-term storage of samples that are mounted with a glass coverslip.

For high-throughput approaches, the silicone gasket can be removed before starting the staining. This allows for the processing of several slides at once in a staining jar. As an alternative to low-throughput experiments, the silicone gasket can be left in position, allowing for separate staining in each well.

Advantages

• Ideal for long-term storage
• High-throughput screening possible

Limitations

• No high-resolution microscopy during cell cultivation

Fluorescence microscopy of immunostained MCF-7 cells in the 8 Well Chamber, removable. Green: alpha-Tubulin. Red: F-actin (phalloidin). Blue: nuclei (DAPI). Widefield fluorescence images were taken with a 20x objective.