The ibidi µ-Slide 8 well allows standard immunofluorescence protocols to be employed without the use of coverslips in an all-in-one chamber. All steps (i.e., cell cultivation, fixation, staining and imaging) are carried out in the classic open well geometry. After staining, the sample can be observed through the coverslip-like bottom using high resolution microscopy. The closely fitting lid prevents evaporation and allows for long-term assays.
Closely fitting lid Suitable for high resolution microscopy
Other Possible Applications
To test different compound concentrations, e.g., in stem cell development
Lee Shu Ying, National University of Singapore, Singapore
"As a microscopy core facility, we have to recommend the most suitable imaging plates to our users for their experiments. The µ-Slide 8 Well is our best seller amongst the other wonderful platforms that ibidi carries.
We had multiple experiments where the cells/bacteria were not adhering well to glass coverslips, but grew perfectly on the ibiTreat surface. Confocal images turned out great as well. Our users also love how the cover fits snugly for ease of handling."
Lee Shu Ying National University of Singapore Yong Loo Lin School of Medicine Singapore
Madeleine Zerbato, Brigham Regenerative Medicine Center, Cambridge, USA
"The ibidi µ-Slide 4 Well and µ-Slide 8 Well that I am working with are actually the best products I have tested so far for cultivating D3 mouse embryonic cell lines. They attach well to the treated slide (ibiTreat µ-Slides), and the resulting morphology is very similar to the one they have on regular plastic. That is something very unique to those microscopic slides, since D3 mESCs do not attach to glass and form embryoid-like structures. I tested other non-glass slides from other manufacturers but the ibiTreat µ-Slides are the only ones that give me a physiological-like morphology.”
Madeleine Zerbato Brigham Regenerative Medicine Center Cambridge, USA
"I used the µ-Slide 8 Well in the glass version and in the plastic version with ibiTreat. They were perfect for my experiment—I was performing live cell imaging on 3D cell culture. I found that the Matrigel adhered very well to both slides. The coverslip thickness of the base resulted in superb imaging. Additionally, I noticed that the lid was very secure compared with other chamber slides I have used in the past. I am very happy with the product and have recommended it to other colleagues.”
Stefanie Kirchberger, Children's Cancer Research Institute, Vienna, Austria
"We tested the µ-Slide 2 Well and µ-Slide 8 Well in the uncoated version for the confocal microscopy of fish larvae. Both types of slides worked fine on our confocal microscope. We have already ordered another bunch of those slides for the lab!"
Stefanie Kirchberger Children's Cancer Research Institute Vienna, Austria
Ivan Bedzhov, University of Cambridge, Gurdon Institute, Cambridge, United Kingdom
“We looked for an imaging-compatible material that supports embryo attachment. This led us to establish that we can successfully grow and image mouse embryos on ibiTreat μ-Plates 24 Well and µ-Slides 8 Well from ibidi, which have optical properties similar to glass, and allow efficient blastocyst attachment. Thus, ibidi slides and plates are now an indispensable part of our in vitro culture methodology.“
Ivan Bedzhov University of Cambridge Gurdon Institute Cambridge United Kingdom
Owen McCarty, PhD, Oregon Health & Science University, Portland, USA
“The ibidi platforms have opened new possibilities for our group to utilize small volumes of blood to characterize the cell biology of platelets and white blood cells. Moreover, we have greatly benefited from collaborating with the technical representatives at ibidi to discuss ways to multiplex the ibidi platforms to address our experimental questions.”
Owen McCarty, PhD Associate Professor Department of Biomedical Engineering Oregon Health & Science University Portland, Oregon USA
"I plated and transfected cells on the ibidi µ-Slide 8 Well alongside the regular chambers we normally use and they were very good. In fact, I particularly liked the lids which seem more secure than our regular 8-well chambers and the fact that they are 'slide' sized with space for writing a label. Another bonus was the individual packaging which gives me greater confidence on sterility particularly when not all the chambers in a pack of 8 are used at once. So all in all great."
Joanne Marrison Imaging and Cytometry Laboratory (B/K051) Bioscience Technology Facility, Department of Biology University of York, York United Kingdom