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MV 18: Immunofluorescence Using the µ-Slide VI 0.4


In this video, we demonstrate an immunofluorescence protocol using the ibidi µ-Slide VI 0.4.


This all-in-one chamber simplifies immunofluorescence protocols by combining cell culture, staining, and imaging in one slide—using only small numbers of cells and minimal reagent volumes.

Key Features of the µ-Slide VI 0.4

  • Six parallel channels for efficient, comparative experiments
  • Homogeneous cell distribution over the channel surface
  • Only 30 µl per channel—cost-effective with reduced reagent consumption
  • Compatible with high-resolution and super-resolution microscopy
  • Suitable for static or flow conditions with defined shear stress

Applications

  • Immunofluorescence assays
  • Live cell imaging under static or flow conditions
  • Parallel screenings using multichannel pipettes
  • High-resolution and super-resolution fluorescence microscopy (e.g., STED, confocal)

Detailed product information: µ-Slide VI 0.4