MV 41: Advanced ibidi Technologies:
Micropatterning, 3D Cell Culture and Flow Applications

Dr. Jan Schwarz gave a talk about ibidi's Micropatterning, 3D Cell Culture and Flow Applications as part of the virtual Aurox Conference on Microscopy, which was held April 7-8, 2020.

Abstract

In recent years, 3D organoid- and spheroid-based assays have drastically improved our understanding of cellular behavior in vitro and in vivo. However, maintaining sufficient nutrient supply in 3D cell cultures, combined with achieving high-quality imaging over extended periods, remains challenging—limiting their use in high-throughput applications.

We developed a perfusable channel system with numerous cell aggregate adhesion sites on a highly passivated coverslip. Spheroids can be directly generated through the injection of cell suspension via a self-sorting process. Alternatively, preformed spheroids or organoids can be homogenously tethered in large numbers. The high optical quality of the imaging bottom, combined with localized tethering of cell aggregates, enables high-throughput live-cell microscopy together with defined shear stress, metabolite analysis, toxicological screenings, and co-culture of multiple spheroid types.

Find out detailed information on ibidi Micropatterning, Cell Culture Under Flow, and 3D Cell Culture!