MV 32: Tube Formation Assays Using the µ-Slide 15 Well 3D

In this video, we demonstrate how to perform a tube formation assay (angiogenesis assay) using the ibidi µ-Slide 15 Well 3D—a complete solution for endothelial tube formation experiments and video microscopy.

You’ll learn how to go from sample preparation to image analysis in just a few steps:

• Seed endothelial cells on top of a thin 3D gel layer (e.g., Matrigel®).
• Watch them form capillary-like structures.
• Record high-quality images for quantitative analysis.

The µ-Slide 15 Well 3D provides a number of key advantages:

• Only 10 µl gel required per well (saves costs and material).
• No gel meniscus formation—cells grow in a uniform optical plane.
• Excellent optical quality for phase contrast and fluorescence microscopy.
• Ideal for angiogenesis, sprouting assays, 3D culture, and immunofluorescence staining.

With its unique “well-in-a-well” design, the µ-Slide 15 Well 3D enables reproducible results in low-volume experiments, making it a powerful tool for angiogenesis research.

Detailed product information: µ-Slide 15 Well 3D