All Videos in Numerical Order

MV 18: Immunofluorescence Using the µ-Slide VI
MV 20: The ibidi Pump System
MV 21: Long-Term Cell Culture Under Perfusion
MV 26: Cell Culture and Microscopy Using the µ-Slide 8 Well
MV 27: Cell Culture and Microscopy Using the µ-Slide I Luer
MV 28: Cell Culture and Microscopy Using the µ-Dish 35 mm, high
MV 30: Live Cell Imaging in Multiwell Plates
MV 31: Live Cell Imaging in ibidi µ-Slides
MV 32: Tube Formation Assays Using the µ-Slide 15 Well 3D
MV 33: Wound Healing Assays Using the Culture-Insert 2 Well
MV 34: 48 Hours Live Cell Imaging of MDCK Cells
MV 35: Tube Formation Assay of HMEC Cells
MV 37: Chemotaxis Assays Using the µ-Slide Chemotaxis
MV 41: Advanced ibidi Technologies: Micropatterning, 3D Cell Culture and Flow Applications
MV 43: Handling the 8 Well Chamber, removable
MV 46: How to use the µ-Slide Spheroid Perfusion
Z-Stack Through a Spheroid Before and After Clearing in the µ-Slide Spheroid Perfusion
MV 48: Filling of the µ-Slide I Luer
MV 49: Medium Exchange in the µ-Slide I Luer
MV 50: Filling of the µ-Slide VI 0.4
MV 51: Medium Exchange in the µ-Slide VI 0.4
MV 52: Handling the µ-Slide 8 Well high
MV 53: Handling the µ-Plate 24 Well
MV 54: Handling the µ-Plate 96 Well Square
MV 55: Handling the µ-Plate 96 Well Round
MV 56: Handling the µ-Plate 384 Well Glass Bottom
MV 57: Handling the µ-Slide I Luer 3D
MV 58: Handling the µ-Slide Spheroid Perfusion
MV 59: Handling the µ-Plate 6 Well
MV 60: The Principle of Phase Contrast Microscopy – Simply Explained
MV 61: Optimization of Phase Contrast Microscopy – Simply Explained
MV 62: The ibidi µ-Patterning Technology
MV 63: Handling the µ-Plate 24 Well for Membrane Inserts
Motility in Microgravity—The ibidi µ-Slide I Luer0.8 on the ISS

MV 46: How to use the µ-Slide Spheroid Perfusion

The ibidi µ-Slide Spheroid Perfusion is the ideal solution for long-term 3D spheroid/organoid cultivation and high-end microscopy.

It consists of 3 x 7 flat-bottomed wells, which are connected through a channel above. Each well forms its own niche, where the specimen is cultured.

When applying perfusion through the channel, fresh medium continuously diffuses to the specimen. This ensures optimal nutrition and oxygen diffusion throughout the experiment without that the specimen is exposed to significant shear forces.

The setup guarantees maximum of viability, but with minimum of shear stress for spheroids, organoids, or tissue.

Detailed product information: µ-Slide Spheroid Perfusion
Find more information on 3D Cell Culture here.

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All Videos in Numerical Order

µ-Slide 8 Well high

µ-Slide I Luer

µ-Plate 96 Well Round

µ-Slide 8 Well high Glass Bottom

µ-Dish 35 mm, high

MV 46: How to use the µ-Slide Spheroid Perfusion

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