MV 75: Fluorescence Staining in the µ-Slide VI

In this video, we demonstrate an immunofluorescence protocol using the ibidi µ-Slide VI ^0.4. For detailed step-by-step instructions, please refer to Application Note 09: Fluorescence Staining Using a µ-Slide VI (PDF).

This all-in-one chamber simplifies immunofluorescence protocols by combining cell culture, staining, and imaging in one slide—using only small numbers of cells and minimal reagent volumes.

Key Features of the µ-Slide VI ^0.4

• Six parallel channels for efficient, comparative experiments
• Homogeneous cell distribution over the channel surface
• Only 30 µl per channel—cost-effective with reduced reagent consumption
• Compatible with high-resolution and super-resolution microscopy
• Suitable for static or flow conditions with defined shear stress

Applications

• Immunofluorescence assays
• Live cell imaging under static or flow conditions
• Parallel screenings using multichannel pipettes
• High-resolution and super-resolution fluorescence microscopy (e.g., STED, confocal)

Detailed product information: µ-Slide VI ^0.4, AN 09: Fluorescence Staining Using a µ-Slide VI (PDF)