MV 61: Optimization of Phase Contrast Microscopy
Simply Explained

Achieving high-quality phase contrast images of unstained cells requires precise microscope alignment. A properly adjusted Koehler illumination is essential for sharp focus and even sample illumination—both prerequisites for optimal contrast. A precise alignment of the annular ring and phase ring within the optical pathway is essential as well, as misalignment—caused by mechanical shifts or optical disturbances such as the meniscus effect in cell culture fluids—can significantly weaken the phase contrast, making cellular details harder to observe.

Therefore, this video will provide a step-by-step protocol on how to align Koehler illumination, match the annular to the phase ring and choose the right labware minimizing or avoiding a meniscus.