MV 34: 48 Hours Live Cell Imaging of MDCK Cells
Watch these happy healthy cells grow and proliferate!
Epithelial MDCK (Madin Darby Canine Kidney) cells were cultivated and imaged for 48 h under physiological conditions.
Material and Methods:
- Passage 12 MDCK cells were cultured in DMEM supplemented with 10% fetal calf serum (FCS) and seeded in the ibidi µ-Slide VI 0.4
- Phase contrast, time lapse microscopy was performed on a Nikon Eclipse TiE inverted microscope with 20x objective for 48 h with a time intervall of 10 min and a total of 289 frames.
- Temperature (37 °C) and Humidity (75%) were controlled using the ibidi Stage Top Incubator