All Videos in Numerical Order

MV 18: Immunofluorescence Using the µ-Slide VI
MV 20: The ibidi Pump System
MV 21: Long-Term Cell Culture Under Perfusion
MV 26: Cell Culture and Microscopy Using the µ-Slide 8 Well
MV 27: Cell Culture and Microscopy Using the µ-Slide I Luer
MV 28: Cell Culture and Microscopy Using the µ-Dish 35 mm, high
MV 30: Live Cell Imaging in Multiwell Plates
MV 31: Live Cell Imaging in ibidi µ-Slides
MV 32: Tube Formation Assays Using the µ-Slide 15 Well 3D
MV 33: Wound Healing Assays Using the Culture-Insert 2 Well
MV 34: 48 Hours Live Cell Imaging of MDCK Cells
MV 35: Tube Formation Assay of HMEC Cells
MV 37: Chemotaxis Assays Using the µ-Slide Chemotaxis
MV 41: Advanced ibidi Technologies: Micropatterning, 3D Cell Culture and Flow Applications
MV 43: Handling the 8 Well Chamber, removable
MV 46: How to use the µ-Slide Spheroid Perfusion
Z-Stack Through a Spheroid Before and After Clearing in the µ-Slide Spheroid Perfusion
MV 48: Filling of the µ-Slide I Luer
MV 49: Medium Exchange in the µ-Slide I Luer
MV 50: Filling of the µ-Slide VI 0.4
MV 51: Medium Exchange in the µ-Slide VI 0.4
MV 52: Handling the µ-Slide 8 Well high
MV 53: Handling the µ-Plate 24 Well
MV 54: Handling the µ-Plate 96 Well Square
MV 55: Handling the µ-Plate 96 Well Round
MV 56: Handling the µ-Plate 384 Well Glass Bottom
MV 57: Handling the µ-Slide I Luer 3D
MV 58: Handling the µ-Slide Spheroid Perfusion
MV 59: Handling the µ-Plate 6 Well
MV 60: The Principle of Phase Contrast Microscopy – Simply Explained
MV 61: Optimization of Phase Contrast Microscopy – Simply Explained
MV 62: The ibidi µ-Patterning Technology
MV 63: Handling the µ-Plate 24 Well for Membrane Inserts
Motility in Microgravity—The ibidi µ-Slide I Luer0.8 on the ISS

MV 35: Tube Formation Assay of HMEC Cells

Watch how endothelial cells form tubes from single cells within just 24 hours!

Human Microvascular Endothelial Cells (HMEC-1) were cultivated and imaged for 24 h under physiological conditions to visualize tube formation.

Material and Methods:

Primary HMEC-1 cells cultured in Endothelial Cell Growth Medium (Promocell)
Seeded in the ibidi µ-Slide 15 Well 3D on Matrigel® (BD)
Phase contrast, time-lapse microscopy on a Nikon Eclipse TiE inverted microscope (10x objective)
Imaging for 24 h with 5 min intervals
Controlled conditions: 37 °C + humidity using the ibidi Stage Top Incubator

Detailed product information: µ-Slide 15 Well 3D

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All Videos in Numerical Order

µ-Slide 8 Well high

µ-Slide I Luer

µ-Plate 96 Well Round

µ-Slide 8 Well high Glass Bottom

µ-Dish 35 mm, high

MV 35: Tube Formation Assay of HMEC Cells

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