µ-Plate Angiogenesis 96 Well

Applications

• High throughput tube formation assays
• 3D cell culture
• Immunofluorescence staining
• Sprouting assays
• Live cell imaging of attached cells

Specifications

Technical drawings and details are available in the Instructions (PDF).

Technical Features

• Meets ANSI/SLAS (SBS) standards
• 4 mm well within a 5 mm well
• Geometry enables pipetting of a homogeneous 0.8 mm thick gel layer*
• Provides homogeneous cell growth
• Compatible with staining and fixation
• Excellent optical properties for microscopy
• Compatible with multi-channel pipettes
• Made of biocompatible plastic material: no glue, no leaking
• Suitable for use with various types of gels* (e.g., Matrigel^®, collagen, and agarose)
• Also available in a slide format: µ-Slide Angiogenesis

*The gel matrix is not included with this product.

Every Cell in Focus:
The Principle of the µ-Plate Angiogenesis 96 Well

The µ-Plate Angiogenesis 96 Well has a specialized geometry for the easy, convenient, and reproducible conduction of tube formation assays. It is also ideal for sprouting assays, immunofluorescence staining, and general 3D cell culture.

After the gel has been pipetted into the inner well and given time to solidify, the cells can be seeded on top of it for tube formation analysis. Due to the “well-in-a-well” technology, the amount of gel needed is reduced to only 10 μl per well, which is 10% of the amount used in regular multiwell plates.

Further, no meniscus is formed. This ensures the formation of a uniformly thick gel matrix on which all cells are in one optical plane, creating reproducible cell culture conditions. The µ-Plate Angiogenesis can be used with all common hydrogel matrices, such as Matrigel^®, collagen gels, agarose gels, and hyaluronic acid gels.

With its 96 wells, the µ-Plate Angiogenesis 96 Well is designed for high throughput assays. For low throughput assays, ibidi provides the μ-Slide Angiogenesis.

The µ-Plate Angiogenesis 96 Well Can Be Used in a Wide Range of Applications

Tube Formation Assay

For a tube formation assay, endothelial cells are seeded on top of a 10 µl gel layer (basement membrane-like surface), where they form capillary-like structures. Typically, Matrigel^® is used. The tube formation ability of the cells can be used to analyze the pro- or anti-angiogenic potential of various drugs.

Phase contrast image showing one well of the µ-Slide Angiogenesis with HUVEC cells on Matrigel^® after 12 hours of incubation during a tube formation assay.

Phase contrast (top) and fluorescence microscopy (bottom) of a single strand composed of HUVEC cells during a tube formation assay in the µ-Slide Angiogenesis. The F-actin cytoskeleton is stained green and the cell nuclei are stained blue.

Sprouting Assay

The sprouting potential of a cell cluster can be conveniently analyzed using the µ-Plate Angiogenesis 96 Well. To carry out a sprouting assay, spheroids or pieces of tissue (e.g., from the aorta), are placed onto or directly in the gel matrix before imaging.

3D Cell Culture

In many cases, a 3D environment more closely resembles an in vivo situation than a 2D cell culture. The µ-Plate Angiogenesis 96 Well is an easy and cost-effective solution for the cultivation and microscopy of cells embedded in gel matrices. The gel layer is directly connected to the medium reservoir above, which allows for fast and easy medium exchange by diffusion. Also, non-adherent cells, such as blood cells or bacteria, can be immobilized for enhanced microscopy access.

Immunofluorescence Staining

For analyzing the outcome of a tube formation or a 3D culture experiment, immunofluorescence staining can be performed in the µ-Plate Angiogenesis 96 Well. The cells can be fixated and stained directly on or in the gel, then imaged using high-resolution microscopy.

Focusing Cells

When using less than 10 µl of gel to fill the inner well of the µ-Plate Angiogenesis 96 Well, small numbers of cells can be cultivated and focused on a soft gel surface.

Low Volume Microscopy Chamber

The µ-Plate Angiogenesis 96 Well can also be used as a low-volume microscopy chamber without any gel, which is useful for experiments where several wells with a low volume are required.