*The gel matrix is not included with this product.
Every Cell in Focus: The Principle of the µ-Plate Angiogenesis 96 Well
The µ-Plate Angiogenesis 96 Well has a specialized geometry for the easy, convenient, and reproducible conduction of tube formation assays. It is also ideal for sprouting assays, immunofluorescence staining, and general 3D cell culture.
After the gel has been pipetted into the inner well and given time to solidify, the cells can be seeded on top of it for tube formation analysis. Due to the “well-in-a-well” technology, the amount of gel needed is reduced to only 10 μl per well, which is 10% of the amount used in regular multiwell plates.
Further, no meniscus is formed. This ensures the formation of a uniformly thick gel matrix on which all cells are in one optical plane, creating reproducible cell culture conditions. The µ-Plate Angiogenesis can be used with all common hydrogel matrices, such as Matrigel®, collagen gels, agarose gels, and hyaluronic acid gels.
With its 96 wells, the µ-Plate Angiogenesis 96 Well is designed for high throughput assays. For low throughput assays, ibidi provides the μ-Slide Angiogenesis.
The µ-Plate Angiogenesis 96 Well Can Be Used in a Wide Range of Applications
Tube Formation Assay
For a tube formation assay, endothelial cells are seeded on top of a 10 µl gel layer (basement membrane-like surface), where they form capillary-like structures. Typically, Matrigel® is used. The tube formation ability of the cells can be used to analyze the pro- or anti-angiogenic potential of various drugs.
Find more detailed information here:
For practical details, please watch the ibidi movie Performing an Angiogenesis Assay (MV 15). In our Application Notes, you will find detailed information on the setup, optimization, data analysis, and interpretation of tube formation assays:
Phase contrast image showing one well of the µ-Slide Angiogenesis with HUVEC cells on Matrigel® after 12 hours of incubation during a tube formation assay.
Phase contrast (top) and fluorescence microscopy (bottom) of a single strand composed of HUVEC cells during a tube formation assay in the µ-Slide Angiogenesis. The F-actin cytoskeleton is stained green and the cell nuclei are stained blue.
The sprouting potential of a cell cluster can be conveniently analyzed using the µ-Plate Angiogenesis 96 Well. To carry out a sprouting assay, spheroids or pieces of tissue (e.g., from the aorta), are placed onto or directly in the gel matrix before imaging.
3D Cell Culture
In many cases, a 3D environment more closely resembles an in vivo situation than a 2D cell culture. The µ-Plate Angiogenesis 96 Well is an easy and cost-effective solution for the cultivation and microscopy of cells embedded in gel matrices. The gel layer is directly connected to the medium reservoir above, which allows for fast and easy medium exchange by diffusion. Also, non-adherent cells, such as blood cells or bacteria, can be immobilized for enhanced microscopy access.
For analyzing the outcome of a tube formation or a 3D culture experiment, immunofluorescence staining can be performed in the µ-Plate Angiogenesis 96 Well. The cells can be fixated and stained directly on or in the gel, then imaged using high-resolution microscopy.
When using less than 10 µl of gel to fill the inner well of the µ-Plate Angiogenesis 96 Well, small numbers of cells can be cultivated and focused on a soft gel surface.
Low Volume Microscopy Chamber
The µ-Plate Angiogenesis 96 Well can also be used as a low-volume microscopy chamber without any gel, which is useful for experiments where several wells with a low volume are required.
Sam Noppen, Rega Institute for Medical Research, Leuven, Belgium
"I used the ibidi µ-Plate Angiogenesis 96 Well for bioluminescence measurements. The plate performed exceptionally well for this specific application, as it reduced my sample volume drastically and the optical quality was just superb."
Sam Noppen Rega Institute for Medical Research, KU Leuven Leuven Belgium