µ-Plate Angiogenesis 96 Well

Applications:

• Tube formation assays
• Sprouting assays
• 3D cell culture
• Immunofluorescence staining
• Cultivation and microscopy of both living and fixed cells

Specifications:

Technical Features:

• Meets ANSI/SLAS (SBS) Standards
• ibidi Polymer Coverslip (180 µm) for high resolution microscopy

Angiogenesis Assays in vitro

Cells on gel matrices can be used to monitor their ability to form new vascular structures. Tube formation assays are carried out by seeding single cells onto the gel matrix and observing characteristic patterns. To carry out sprouting assays, either spheroids or pieces of tissue, e.g., from the aorta, are placed onto the gel matrix. Both these types of assays require that the underlying gel layer be of uniform thickness.

µ-Plate Angiogenesis 96 well vs. Standard 96 well Plate

µ-Plate Angiogenesis
96 Well

1. Planar air-liquid interface:
   good phase contrast all over the observation area
2. Planar gel surface:
   all cells are in one optical plane

Volume of Matrigel: 10 µl

Standard well

1. Meniscus on air-liquid interface:
   poor phase contrast in most of the observation area
2. Mensicus on the gel surface: not possible to focus on all cells simultaneously

Volume of Matrigel: 100 µl

Technical Drawing

Technical drawings and details are available in the Instructions (PDF).