Enhance Your 3D Cell Culture

3D cell cultures, such as spheroids, organoids, or cells in 3D scaffolds, are increasingly adopted for in vitro studies, delivering a more precise representation of the in vivo environment compared to traditional 2D cultures. And now, with advanced cultivation methods such as dynamic flow culture or bioprinting, it is possible to create 3D tissue conditions that are even closer to the biological origin. Hence, 3D cell culture models and advanced cultivation methods lead to more physiologically relevant results with regards to cell viability, gene expression, disease modeling, and drug testing, as well as toxicity studies.

ibidi provides advanced, customizable solutions for the creation and image-based analysis of 3D cell models. Our modular systems are designed to simplify the complexity underlying cutting-edge 3D cell-based assays. By integrating various components of the ibidi products, researchers can effortlessly manage complex designs, enhancing the robustness and efficiency of their scientific investigations. With the ibidi µ-Slides / µ-Dishes / µ-Plates, the ibidi Pump System, or the ibidi Stage Top Incubators, you can take your research to the next level and achieve more accurate and reliable results.

Experimental Workflow

Discover the solutions for all your experimentation needs, from sample preparation to 3D cell cultivation, imaging, and analysis in the 3D Cell Culture Workflow chapter.

Systems and Applications

3D cell culture experiments require robust setups that work with absolute precision and reliability. The ibidi products, including the µ-Slidesµ-Dishesµ-Plates, the ibidi Pump System, and the ibidi Stage Top Incubators, are designed for performing and analyzing 3D cell culture assays under physiologic conditions. Thanks to their flexibility and modularity, they can be easily adapted to your experimental needs. 

ibidi Blog Article

Do you want to get an overview about the big variety of 3D Cell Culture Models? Then read the ibidi Blog.


Selected Publications Using 3D Cell Culture Models

Human blastoids were cultured on a 3D matrix using the µ-Slide 8 Well.
Karvas RM, Zemke JE, Ali SS, Upton E, Sane E, Fischer LA, Dong C, Park KM, Wang F, Park K, Hao S, Chew B, Meyer B, Zhou C, Dietmann S, Theunissen TW. 3D-cultured blastoids model human embryogenesis from pre-implantation to early gastrulation stages. Cell Stem Cell., 2023, 10.1016/j.stem.2023.08.005.
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3D sandwich cultures of squameous cell carcinoma cells were done in the µ-Plate 96 Well 3D.
E. Hoque Apu, S.U. Akram, J. Rissanen, H. Wan and T. Salo. Desmoglein 3 – Influence on oral carcinoma cell migration and invasion. Experimental Cell Research, 2018, 10.1016/j.yexcr.2018.06.037.
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The 12 Well Chamber, removable was used for the cultivation of 3D human acinar ductal metaplasia cells.
da Silva, L., Jiang, J., Perkins, C. et al. Pharmacological inhibition and reversal of pancreatic acinar ductal metaplasia. Cell Death Discov., 2022, 10.1038/s41420-022-01165-4.
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3D cell culture of human breast epithelial MCF10A cells was done in the µ-Slide 8 Well.
H. Grobe, A. Wüstenhagen, C. Baarlink, R. Grosse and K. Grikscheit. A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions. PloS one, 2018, 10.1371/journal.pone.0194716.
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