Is the coverslip thickness important when using inverted microscopy, and are different thicknesses available?
Yes, the thickness of the coverslip is a crucial aspect of imaging quality. Most objective lenses for microscopy are made for a coverslip thickness of 0.17 mm (170 µm, No. 1.5). A thinner or thicker substrate will require correction collars on the objective lenses to prevent the formation of blurred images due to spherical and chromatic aberrations. Therefore, in addition to the standard No. 1.5 thickness, further coverslip thicknesses are available:
| No. | Thickness |
| No. 0 | 0.08 - 0.12 mm |
| No. 1.0 | 0.13 - 0.16 mm |
| No. 1.5 | 0.16 - 0.19 mm |
| No 1.5H | 0.165 – 0.175 mm |
| No. 2.0 | 0.19 - 0.23 mm |
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