Frequently Asked Questions

ibidi Labware | Microscopy

Can the sticky-Slide be detached from its substrate mounting?

Does autofluorescence occur when using the ibidi Polymer Coverslip?

What is the stiffness / Young's Modulus of the ibidi Polymer and Glass coverslips?

Which components of cell culture media can cause background fluorescence?

When discussing risk management, which hazards should I be aware of when using ibidi labware products?

Is the flat design of the µ-Slide 18 Well - Flat also compatible with upright microscopes?

Do cells seeded in 3D gel get enough nutrients?

Which ibidi products are compatible with differential interference contrast (DIC) microscopy?

How does the optical quality of the ibidi Polymer Coverslip compare to that of glass?

What is the Abbe number / material dispersion of the ibidi Polymer Coverslip and the Glass Coverslip bottom?

What is the thickness of the ibidi bottom materials?

What is the refractive index of the ibidi Polymer Coverslip and the Glass Coverslip bottom?

Is the coverslip thickness important when using inverted microscopy, and are different thicknesses available?

When using fluorescent microscopy, how much light can cells be exposed to during live cell imaging without being damaged?

What are the microscopy properties of the ibidi Polymer Coverslip bottom?

Is the ibidi labware compatible with super-resolution microscopy (STED, SIM, (F)PALM, (d)STORM)?

Which immersion oils are compatible with the ibidi labware products?

Which ibidi labware would you recommend for confocal microscopy?

Does the Bioinert coating swell?

Why is my ibidi sticky-Slide leaking?

What are the passivation properties of the ibidi Bioinert surface?

Do gold particles attach to the ibidi labware?

How can the meniscus be minimized in the ibidi µ-Plate 24 Well?

Why is the grid in the gridded µ-Dishes and µ-Slides located on the top and not on the bottom of the coverslip?

Is ibiSeal compatible with phase contrast imaging?

Is ibiSeal compatible with differential interference contrast (DIC) imaging?

How long can cells be cultured in the µ-Slide 18 Well - Flat?

Can I use the 3 Well Chamber, removable without the 15 mm glass coverslip?

Is the silicon gasket of the Immunofluorescence Chamber Slides ( 3 Well | 8 Well | 12 Well Chamber, removable) reusable?

Which mounting medium is recommended for mounting glass slides and coverslips in the Immunofluorescence Chamber Slides 3 Well |

Can suspension cells (e.g., lymphoma cells) be attached to fibronectin?

Is it possible to perform independent experiments in the minor wells of a µ-Slide 2 Well Co-Culture?

Can cells move across the small barriers between the wells in a µ-Slide 2 Well Co-Culture?

Which mounting solution is optimal for immunofluorescence assays when using the ibidi labware?

How do I properly fill the µ-Slide 2 Well Ph+ or the µ-Slide 4 Well Ph+?

What does the "Ph+" in the µ-Slide 2 Well Ph+ and the µ-Slide 4 Well Ph+ stand for?

How can the evaporation of medium be prevented?

What is your recommendation for reusing μ-Slides, µ-Dishes, or µ-Plates?

My µ-Slides/µ-Dishes show scratches on the bottom. Where do these come from and how can I prevent them?

Do cells grow directly on the ESS surface?

What is the meaning of “tissue culture-treated glass”?

What is the ibiTreat surface and what experiments can you use it for?

Are the µ-Slides, µ-Dishes, and µ-Plates biocompatible?

What are the dimensions of the ibidi labware products?

Is there a chance of chemicals leaching into my assay when using ibidi polymer products?

Which surface coatings are offered by ibidi?

Which solvents and chemicals are compatible with the ibidi labware?

Is there a cleaning protocol for ibidi glass bottom products?

Can you place ibidi Culture-Inserts on a pre-coated surface?

What level of gas exchange/permeability normally occurs in ibidi polymer coverslips? Do they actually permit gas exchange at all?

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