We recommend the ibidi Mounting Medium, a glycerol-based mounting solution that is optimized for immunofluorescence stainings using the ibidi µ-Slides, µ-Dishes, µ-Plates. The ready-to-use, formulation contains an anti-fade reagent that helps reduce photobleaching of fluorochromes during fluorescence imaging and provides a refractive index that supports improved optical clarity and imaging quality by reducing light scattering and spherical aberrations.

In general, mounting media are used in immunofluorescence assays to:

• Preserve fluorescence signals by reducing photobleaching (anti-fade effect)
• Improve optical imaging quality through refractive index (RI) matching between sample, coverslip, and objective
• Protect and stabilize samples during imaging and long-term storage

Different types of mounting media can be used, depending on the application:

• Non-hardening mounting media remain liquid after mounting and are ideal for standard applications and provide sample flexibility. These media are often water- or glycerol-based, making them generally easier to handle and less hazardous than solvent-based hardening media.
• Hardening mounting media cure after application and permanently seal the sample for long-term storage and archiving.

The ibidi Mounting Medium is classified as a water-based, glycerol-containing, non-hardening mounting medium. It is particularly suitable for large- and small-volume applications using the ibidi µ-labware. When using the the Removable Chamber 3 Well, Removable Chamber 8 Well, or Removable Chamber 12 Well, we recommend a hardening mounting medium for long-term storage and the ibidi Mounting Medium for short-term use.