Join Us for Our 15th ibiTea on October 15, 2025
The Dos and Don’ts of Fluorescence Light Microscopy
Part 2
Presented by Dr. Roland Nitschke, Life Imaging Center, Albert Ludwigs University Freiburg, Germany
We cordially invite you to our 15th ibiTea webinar, where you’ll learn about the relevance of best practices in light microscopy from a leading scientist in the field of fluorescence and live cell imaging. This webinar will address every level of user knowledge and will cover topics ranging from sample preparation and imaging to microscopy hardware settings.
Are you ready to learn more about best practices in light microscopy? If so, grab a cup of tea and join our scientific discussion on:
October 15, 2025, 4 pm Munich CEST
(10 am New York EDT | 3 pm London BST)
Click here to check the time in your own country.
The number of participants is limited, so please reserve your seat now.
ibiTea Topic Outline
In the 15th ibiTea, Roland Nitschke will continue presenting key parameters that influence the quality of images acquired with a fluorescence microscope. He will discuss important experimental and instrumental requirements and settings essential for performing quantitative fluorescence microscopy experiments, including tools for evaluating the condition and performance of your microscope.
Topics will include how to choose the right instrument for your imaging needs—whether it’s a widefield microscope or sectioning microscopes such as spinning disk, single-point confocal scanners, light-sheet, or TIRF microscopy.
The discussion will cover the impact of various factors, including the mounting or embedding medium, refractive index, and cover glass, as well as adjustments using the objective correction collar. Additionally, we will examine the stability and linearity of light source intensity, the uniformity of illumination across the imaging field, and the relationship between camera pixel size and image pixel size. Other important considerations include detector saturation and image offset, focal depth, and image resolution in the x, y, and z dimensions. The session will also address image co-registration and the prerequisites for colocalization analysis and ratio imaging
Ratio imaging of the dynamic distribution of a cytosolic CFP/YFP FRET construct (Kuppig&Nitschke, J. Microscopy 2007) in HT29 cells. Widefield microscope (Zeiss Observer Z1) equipped with an aplan-apochromat 63x/1.2w objective. Images were captured with the following parameters: 435 nm (CFP) and 514 nm (YFP) excitation with 50 ms exposure (AxioCam MRm) over a period of 129s, emission detected at 530-570 nm, 800x400 pixels.
Speaker
Dr. Roland Nitschke
Life Imaging Center, Albert Ludwigs University Freiburg, Germany
Roland Nitschke developed a passion for fluorescence microscopy during his time at the NIH in the early 1990s. He co-founded GermanBioImaging (GerBI-GMB) and established the trinational network "MIAP" for imaging facilities. For 25 years, he has led the Life Imaging Center in Freiburg, Germany.
Throughout his career, he has collaborated with academic groups, standardization bodies, and industrial partners to improve standardization and quality control in the field. Roland is a member of DIN (NA 027-01-04 AA Microscopes) and the International Organization for Standardization (ISO 172/SC 5 for Microscopes and Endoscopes).
In 2020, Roland spearheaded the establishment of QUAREP-LiMi (Quality Assessment and Reproducibility for Instruments & Images in Light Microscopy). This global initiative, based in Freiburg, aims to create a community-driven and agreed-upon methodology to ensure quality in research utilizing light microscopy.
