Introduction to Cell Culture Under Flow, Shear Stress, and Flow Patterns
Do you want to know…
- … how you can culture endothelial cells under defined shear stress values?
- … what is the basic physical background of laminar flow profiles and shear stress?
- … what equipment is required for a cell culture perfusion setup?
Watch our webinar to find out the answers to these questions.
Endothelial cells in the body normally exist in an environment where they are continuously exposed to the frictional force of the blood flow, called shear stress. Shear stress is a crucial regulator of endothelial cell function in physiological conditions, and disturbed flow and altered shear stress are related to pathologies such as atherosclerosis or thrombosis. Endothelial cells sense shear stress through various receptors and transmit the mechanical shear stress stimuli into intracellular signaling, leading to functional and phenotypic cell changes.
For understanding the physiological conditions in vivo, it is essential to study in vitro models under shear stress. The ibidi Pump System enables the culture and study of endothelial cell function under various shear stress conditions, to address questions including (i) the development of the vasculature, (ii) maintenance of vascular homeostasis, and (iii) the pathogenesis of vascular disease.In this webinar, we will discuss fluidic wall shear stress and different flow patterns, highlighting the physical background and calculation basics. We will also address application examples and flow conditions that can be studied using the ibidi Pump System.
Find Out More
Please find more detailed information about the planning and conduction of cell culture under flow assays here.
Download the whole "Cell Culture Under Flow" Application Guide as a PDF here.
Dr. Irina Hein, Application Specialist
Irina Hein studied Biology and received her doctoral degree in neurobiology at the Max Planck Institute for Neurobiology in Martinsried, Germany. In 2018, she joined ibidi as an Application Specialist for the technical and scientific support of our customers and colleagues worldwide.