Channel slides with different heights, volumes, and coatings specially...
Tube Formation Assay for Studying the Effect of Drugs to Angiogenesis
The tube formation assay based on gel matrices is a powerful and cost effective method for screening the effects of drugs on the angiogenic potential of endothelial cells. This webinar gives a detailed overview on sample preparation, image capture and analysis, as well as available methods to interpret data for angiogenesis investigation.
You will see a complete experiment with measured data using endothelial cells on a Matrigel™ gel matrix. We will point out important experimental details and give advice on how to troubleshoot through possible obstacles.
Preparation of the tube formation assay - Filling the gel into the well to avoid meniscus formation - Seeding cells to initial homogeneous cell density and distribution - How to choose the correct time for taking images
Picture quality for optimal image analysis
Parameters and possible methods of data interpretation and quantification using web-based automated image analysis
Helga Wagner, ibidi GmbH
Helga Wagner studied Biology at the University of Regensburg. Since 2008 she is working in Research & Development of custom specific slides for functional cell based assays at ibidi GmbH. Her focus is on the design and implementation of tube formation assays and flow assays.
Kilian Schramm, Wimasis GmbH
Kilian Schramm studied medical engineering and technology oriented economics at the TU Munich, Germany. Parallel to his studies, he worked for nearly 3 years in the field of data analysis, evaluation and algorithms development. Since 2009, Kilian is managing director at Wimasis, a service company for the analysis of cell-based assays.