Tips for Preparing Microscopy Samples
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When preparing microscopy samples of adherent cells in 2D, the culture vessel being used has to fulfill two requirements: optimal growth conditions for the cells and excellent optical features for perfect imaging.
While the materials used for standard cell culture vessels are optimized for cell attachment and growth, the optics of these plastics is often quite poor and not suitable for high-resolution microscopy.
Therefore, culture vessels made from borosilicate glass are usually preferred for imaging, but have the disadvantage that the properties of glass (such as the material stiffness) are less ideal for cell attachment and growth. In addition, a coating might be necessary for supporting the cell adherence.
At ibidi, we design imaging chambers that combine perfect optics with optimal cell attachment, additionally taking into consideration that the growth, development, and signaling of cultured cells strongly depend on which substrate is used for the seeded cells. Furthermore, we have developed specialized geometries for different cell-based assays and microscopy applications.
In this webinar, we share tips and tricks on how to prepare samples for microscopy. We will give you a comparison of the different surfaces and their impact on cell growth. We share insights on how the geometry of a culture vessel influences cell distribution and imaging quality. Learn how to achieve better images using phase contrast microscopy, plus how to simplify your immunostaining workflow.
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Discover more detailed information on how to culture and prepare cells for microscopy here.
Dr. Peggy Benisch, Head of the ibidi Academy
Dr. Peggy Benisch studied Biology in Bayreuth, Würzburg, and Umeå, Sweden. She received her PhD in stem cell biology at the Orthopedic Center for Musculoskeletal Research in Würzburg. In 2013, she joined ibidi as an Application Specialist for the technical support of our customers worldwide. In 2017, she became the Head of the ibidi Academy, a program for training and conducting practical courses for ibidi partners and users.