Wound Healing and Migration Assays
The wound healing assay is one of the most prominent methods for studying directional cell migration in vitro. Measuring the kinematics of wound healing through the migration of cells requires a cell-free gap between two cell-covered areas. The scratch assay—a typical method for producing cell-free areas—shows poor reproducibility and user-dependent effects.
In this webinar, Elias Horn introduces a new method for wound healing and migration assays based on the ibidi Culture-Insert. The Culture-Insert produces a precisely defined gap size between two cell patches for time-lapse microscopy. He will present the complete workflow of the assay, from sample preparation and live cell imaging to quantitative image analysis. Various application examples will complete this scientific lecture.
The Wound Healing webinar will focus on the following aspects:
- The idea behind Wound Healing and Migration Assays
- Experimental workflow using the ibidi Culture-Inserts
- Comparison to the scratch assay
- Application examples
Elias Horn, ibidi GmbH
Elias Horn works as a product manager for labware and reagents at ibidi. He studied biotechnology at the University of Applied Science, Mittweida. For more than 10 years, he has been working on the development of specific slides and dishes in the Research and Development department at ibidi. Since 2017, he has been part of the ibidi Product Management team.