MV 73: Tips & Tricks for Using Preformed Spheroids in the µ-Slide Spheroid Perfusion

What Is the µ-Slide Spheroid Perfusion?

The µ-Slide Spheroid Perfusion is a microfluidic perfusion slide designed for the long-term culture of spheroids and organoids. It consists of a channel connected to an array of 3 × 7 small wells, where each well forms a defined niche for individual spheroids. These wells are linked via an overlying channel that enables continuous medium perfusion without directly exposing the spheroids to high shear stress. The slide features a Bioinert surface, minimizing unwanted cell adhesion and enabling reliable formation, cultivation, and handling of 3D cell culture. The ibidi #1.5 Polymer Coverslip Bottom provides excellent optical properties and is optimized for high-resolution microscopy, including live cell imaging and fluorescence-based assays.

How Does the µ-Slide Spheroid Perfusion Work?

In use, spheroids can either be generated directly within the wells or transferred from other culture systems. After sealing the system, a controlled flow can be applied through the channel using a the ibidi Pump System, ensuring constant nutrient supply, oxygenation, and removal of waste products. This setup maintains high viability over extended experiments while preserving the structural integrity of the 3D aggregates.

What the µ-Slide Spheroid Perfusion Used For?

The slide is primarily used for the generation and cultivation of spheroids and organoids for a wide range of applications, including organ-on-a-chip applications. Its defined microwell structure enables the formation and analysis of one spheroid per well, ensuring high reproducibility and straightforward experimental handling. The system is optimized for microscopy, allowing direct imaging of individual spheroids for live cell analysis as well as endpoint assays such as immunofluorescence or histology.