I often have trouble loading the observation chamber with the gel mix. How can I improve my method?
Please refer to the detailed troubleshooting section inApplication Note 17.
Chemotaxis Assays
- Is imaging of chemotaxis assays only possible with an inverted microscope, or can an upright microscope also be used?
- Why are video microscopy and cell tracking needed for the chemotaxis assay? Can’t I simply count migrated cells at the end of the experiment?
- Can 3D assays be used for non-adherent cells?
- My cells do not migrate. What can I do?
- How many cells should be tracked in one observation field?
- How long do I have to follow the cell migration with time-lapse microscopy?
- Does the gradient change during the chemotaxis experiment?
- Which data should be shown when presenting chemotaxis results?
- How stable is the gradient over time?
- Does the gel in the µ-Slide Chemotaxis affect the diffusion of chemoattractant molecules and thereby influence the gradient?
- What is a gradient and what is its difference from a concentration profile in chemotaxis assays?
- What is the difference between slice series and track series when using the Chemotaxis and Migration Tool?
- Which chemoattractant should be used as a positive control?
- If all the filling ports on the µ-Slide Chemotaxis chamber are plugged, is it still permeable to CO2 and other gases?
- Which concentration of a chemoattractant should be used?
- Which image is used by the Chemotaxis and Migration Tool for calculating the p-value of the Rayleigh test?
- I often have trouble loading the observation chamber with the gel mix. How can I improve my method?
- Is it possible to do an immunofluorescence staining of cells in a gel matrix using the µ-Slide Chemotaxis?
- How can I do a fibronectin coating in the µ-Slide Chemotaxis?
- What is the recommended seeding density for non-adherent cells for a chemotaxis assay?
- What is the recommended seeding density of adherent cells in a µ-Slide Chemotaxis to conduct a chemotaxis assay?
- Do you have any recommendations on how to avoid bubbles that appear after loading cells in the µ-Slide Chemotaxis?
- Is the ibiTreat surface of the µ-Slide Chemotaxis suitable for culturing HUVECs without coating?
- What is the difference between the Rayleigh test and the Rayleigh test for vector data?
- What is the purpose of the Rayleigh test when analyzing chemotaxis assays?
- Why do all cell tracks start from one single point in the created plots when using the Chemotaxis and Migration Tool?
- How can I plot my chemotaxis data table when using the Chemotaxis and Migration Tool?
- Is an analysis with the Chemotaxis and Migration Tool possible when data points of the cell tracks are missing?
- Which data format is needed for analyzing chemotaxis assays with the Chemotaxis and Migration Tool?
- How do I differentiate directed, chemotactic cell migration from undirected, random migration?
- Do you have a video or other material that illustrates how to use the µ-Slide Chemotaxis?