Chemotaxis Assays

Is imaging of chemotaxis assays only possible with an inverted microscope, or can an upright microscope also be used?
Why are video microscopy and cell tracking needed for the chemotaxis assay? Can’t I simply count migrated cells at the end of the experiment?
Can 3D assays be used for non-adherent cells?
My cells do not migrate. What can I do?
How many cells should be tracked in one observation field?
How long do I have to follow the cell migration with time-lapse microscopy?
Does the gradient change during the chemotaxis experiment?
Which data should be shown when presenting chemotaxis results?
How stable is the gradient over time?
Does the gel in the µ-Slide Chemotaxis affect the diffusion of chemoattractant molecules and thereby influence the gradient?
What is a gradient and what is its difference from a concentration profile in chemotaxis assays?
What is the difference between slice series and track series when using the Chemotaxis and Migration Tool?
Which chemoattractant should be used as a positive control?
If all the filling ports on the µ-Slide Chemotaxis chamber are plugged, is it still permeable to CO2 and other gases?
Which concentration of a chemoattractant should be used?
Which image is used by the Chemotaxis and Migration Tool for calculating the p-value of the Rayleigh test?
I often have trouble loading the observation chamber with the gel mix. How can I improve my method?
Is it possible to do an immunofluorescence staining of cells in a gel matrix using the µ-Slide Chemotaxis?
How can I do a fibronectin coating in the µ-Slide Chemotaxis?
What is the recommended seeding density for non-adherent cells for a chemotaxis assay?
What is the recommended seeding density of adherent cells in a µ-Slide Chemotaxis to conduct a chemotaxis assay?
Do you have any recommendations on how to avoid bubbles that appear after loading cells in the µ-Slide Chemotaxis?
Is the ibiTreat surface of the µ-Slide Chemotaxis suitable for culturing HUVECs without coating?
What is the difference between the Rayleigh test and the Rayleigh test for vector data?
What is the purpose of the Rayleigh test when analyzing chemotaxis assays?
Why do all cell tracks start from one single point in the created plots when using the Chemotaxis and Migration Tool?
How can I plot my chemotaxis data table when using the Chemotaxis and Migration Tool?
Is an analysis with the Chemotaxis and Migration Tool possible when data points of the cell tracks are missing?
Which data format is needed for analyzing chemotaxis assays with the Chemotaxis and Migration Tool?
How do I differentiate directed, chemotactic cell migration from undirected, random migration?
Do you have a video or other material that illustrates how to use the µ-Slide Chemotaxis?

How do I differentiate directed, chemotactic cell migration from undirected, random migration?

Please find detailed information about the data analysis and interpretation of chemotaxis assays in the application chapter “Data Analysis of Chemotaxis Assays".

Chemotaxis Assays

µ-Slide 8 Well high

µ-Slide I Luer

µ-Plate 96 Well Round

µ-Slide 8 Well high Glass Bottom

µ-Dish 35 mm, high

How do I differentiate directed, chemotactic cell migration from undirected, random migration?

How do I differentiate directed, chemotactic cell migration from undirected, random migration?

Name	Provider	What it does	Expiry	Allow (Check all)
LS_CSRF_TOKEN	Cloudflare	Cloudflare sets this cookie to track users’ activities across multiple websites. It expires once the browser is closed. Cloudflare sets this cookie to track users’ activities across multiple websites. It expires once the browser is closed.	Session	Allow
uesign	Zoho	Zoho sets this cookie for the Visitor Live Chat. Zoho sets this cookie for the Visitor Live Chat.	30 days	Allow
_zcsr_tmp	Zoho	Zoho sets this cookie for the login function on the website. Zoho sets this cookie for the login function on the website.	Session	Allow
Shopping cart	PrestaShop	This provides, and keeps the products inside your shopping cart. Deactivating this cookie would stop permitting the orders. This cookie doesn't save any personal Data about any shop client. This provides, and keeps the products inside your shopping cart. Deactivating this cookie would stop permitting the orders. This cookie doesn't save any personal Data about any shop client.	30 days	Allow

Name	Provider	What it does	Expiry	Allow (Check all)
ad_storage	Google	Enables storage, such as cookies (web) or device identifiers (apps), related to advertising. Enables storage, such as cookies (web) or device identifiers (apps), related to advertising.	30 days	Allow
ad_user_data	Google	Sets consent for sending user data to Google for advertising purposes. Sets consent for sending user data to Google for advertising purposes.	30 days	Allow
ad_personalization	Google	Sets consent for personalized advertising. Sets consent for personalized advertising.	30 days	Allow
analytics_storage	Google	Enables storage, such as cookies (web) or app identifiers (apps), related to analytics, e.g. visit duration. Enables storage, such as cookies (web) or app identifiers (apps), related to analytics, e.g. visit duration.	30 days	Allow

Chemotaxis Assays

µ-Slide 8 Well high

µ-Slide I Luer

µ-Plate 96 Well Round

µ-Slide 8 Well high Glass Bottom

µ-Dish 35 mm, high

How do I differentiate directed, chemotactic cell migration from undirected, random migration?

Necessary

Statistics

Marketing