Chemotaxis Assays

Can 3D assays be used for non-adherent cells?

Yes, we think so. The mimicking of in vivo-like migration of non-adherent cells through interstitial tissue is a good argument for using gels in migration assays. We developed a protocol for investigating chemotactic migration of non-adherent dendritic cells in a Collagen I gel. Please refer to Application Note 23 for further details.