HUVECs generally adhere well when using ibidi labware with the ibiTreat (tissue culture-treated) surface, especially, when a growth medium containing 10% fetal calf serum is used. To speed up cell adhesion during chemotaxis assays, however, we recommend using µ-Slides Chemotaxis precoated with Collagen IV. Optionally, it is possible to coat the slides yourself by following the protocol in Application Note 08.